Virology Learning Objectives- Block 2- Uofl Med

Front 1

Which family or families of virus has/have ssDNA composition?

Back 1

Parvo

Front 2

Which family or families of virus has/have circular dsDNA composition?

Back 2

Papilloma and polyoma

Front 3

Which family or families of virus has/have dsDNA composition?

Back 3

Adeno, herpes and pox

Front 4

Which family or families of virus has/have circular ss/dsDNA composition?

Back 4

Hepadna

Front 5

Which family or families of virus has/have circular ssDNA composition?

Back 5

Circo

Front 6

Which family or families of virus has/have ssRNA (+) composition with icosahedral capsid?

Back 6

Picorna, calici, astro, toga, flavi, retro

Front 7

Which family or families of virus has/have ssRNA (+) composition with helical capsid?

Back 7

Corona

Front 8

Which family or families of virus has/have segmented dsRNA (+) composition with icosahedral capsid?

Back 8

Reo

Front 9

Which family or families of virus has/have circular ssRNA (-) composition?

Back 9

Hepatitis D

Front 10

Which family or families of virus has/have ssRNA (-) composition with helical capsid?

Back 10

Rhabdo, filo, paramyxo

Front 11

Which family or families of virus has/have segmented ssRNA (-) composition with helical capsid?

Back 11

Orthomyxo, arena, bunya

Front 12

T/F. Viruses are living.

Back 12

False

Front 13

What is the difference between bacteria and viruses in terms of replication?

Back 13

Bacteria undergo binary fission, while viruses utilize synthetic machinery inside host cells

Front 14

Which antibiotics destroy viruses?

Back 14

None; they are not affected by antibiotics

Front 15

Do viruses contain DNA or RNA? Can they contain both?

Back 15

Either DNA or RNA, but not both

Front 16

How is the virus's dependency on the host cell labeled/termed?

Back 16

Obligate intracellular parasitism

Front 17

What type of capsid do all DNA viruses have?

Back 17

Icosahedral

Front 18

What is a virion?

Back 18

Complete infectious virus particle

Front 19

What is a segmented genome? Give an example.

Back 19

Genome divided into distinct pieces; influenza virus divided into 8 segments

Front 20

What is positive polarity of RNA?

Back 20

Same polarity as mRNA, so genome RNA can be translated in cytoplasm by host cell ribosomes

Front 21

What is negative polarity of RNA?

Back 21

Complementary to mRNA, so genome RNA must be transcribed by viral RNA polymerase to produce viral mRNA

Front 22

What is structural viral protein?

Back 22

Viral genome-encoded protein present in virion (capsid proteins, envelope glycoproteins, HIV and hep B viral reverse transcriptase)

Front 23

What is nonstructural protein?

Back 23

Viral genome encoded proteins not present in virion, but present in virus infected cells (example: herpes viral DNA polymerase)

Front 24

What is viral transcriptase?

Back 24

Uses RNA as template to synthesize RNA (Viral RNA polymerase)

Front 25

What are the 2 different types of viral capsids and which families are more likely to have a type?

Back 25

Icosahedral and helical; dsRNA viruses, most DNA viruses and almost all (+)ssRNA viruses have icosahedral; (-) ssRNA viruses typically have helical

Front 26

Describe the structure of the icosahedral capsid and how it is made?

Back 26

Capsomers (viral polypeptides) arranged in 20 equilateral triangular faces & 12 vertices with 5-fold symmetry; formed by self-assembly or viral proteins prior to packaging of viral genome.

Front 27

What is the function of the capsid?

Back 27

To protect the viral genome, facilitate attachment to cells, and confer antigenicity

Front 28

Describe the structure of the helical capsid.

Back 28

Identical protomers arranged in a helix with the nucleic acid between the turns; positive charged protein to negative charged phosphodiester backbone of RNA

Front 29

Describe the composition of viral envelopes.

Back 29

Host derived lipid bilayer with viral glycoprotein (structural proteins).

Front 30

Which virus families typically have viral envelopes?

Back 30

(-) ssRNA viruses (Paramyxo, Rhabdo, Filo); some (+) ssRNA families (Retro, Toga, Flavi and Corona); DNA viruses include Herpes and Hepadna

Front 31

Do poxviruses have viral envelopes?

Back 31

No; they have some lipids but are NOT really lipid-enveloped

Front 32

How can viral envelopes be degraded?

Back 32

By lipid solvents, heat or low pH

Front 33

What is the consequence of the acid-labile property of viral envelopes?

Back 33

Infectivity of lipid-enveloped viruses lost more rapidly and destroyed by passage through the stomach; as a result, enveloped viruses can not pass through the GI tract or be transmitted by the fecal-oral route

Front 34

T/F. Only naked-icosahedral viruses have the potential to survive passage through the stomach to replicate in small intestine and be efficiently transmitted as contagious virus by fecal-oral route.

Back 34

True

Front 35

How are contagious lipid-enveloped viruses transmitted?

Back 35

Via respiratory secretions

Front 36

What are the peak seasons for contagious lipid-enveloped viruses?

Back 36

Winter-spring

Front 37

What are the peak seasons for naked icosahedral RNA viruses?

Back 37

Summer-fall

Front 38

What are the important viral proteins in eliciting neutralizing antibody with immunization and natural infection?

Back 38

In lipid-enveloped viruses= glycoproteins in the lipid-envelope
In naked icosahedral viruses = viral capsid proteins