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25 Cards in this Set
- Front
- Back
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what causes the different types of CPE?
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different viruses in different cells
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what CPE's can be caused by viruses?
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inclusion bodies, multinucleate syncytia
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what are inclusion bodies?
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viral factories
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what types of inclusion bodies are there?
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intracytoplasmic acidophilic, intranuclear acidophilic, perinuclear intracytoplasmic acidophilic, intranuclear basophilic
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what type of inclusion bodies does poxvirus cause in prairie dogs?
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intracytoplasmic eosinophilic inclusion bodies
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what type of inclusion bodies does pacheco's parrot disease virus (herpesvirus) cause in parrots?
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intranuclear eosinophilic inclusion bodies
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what type of inclusion bodies does rabies virus (rhabdovirus) cause?
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intracytoplasmic eosinophilic inclusion bodies (negri bodies)
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what are negri bodies?
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the inclusion bodies formed by rabies
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what type of inclusion bodies does canine distempter virus (paramyxovirus) cause in canines?
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intranuclear and intracytoplasmic eosinophilic inclusion bodies
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what type of inclusion bodies does peste des petits ruminant virus (paramyxovirus) cause in goats?
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intranuclear eosinophilic inclusion bodies and multinucleate syncytia
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how are multinucleate syncytia formed?
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some enveloped viruses have fusion proteins in the envelope and during infection, new fusion proteins are translated in cells where they are transported to cell surface and expressed on cell membrane so that budding viruses can pick up these proteins when picking up envelope from cell membranes, but these fusion proteins can also cause fusion of infected cell with neighboring uninfected cell so that hte virus can spread from cell to cell without exiting cell
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what is the embryonated egg useful for?
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large scale propagation of virus (influenza and rabies vaccine) & small scale propagation for identiication of virus (inoculate egg, wait for CPE or death, harvest, perform in vitro assay to id) particularly for poultry viruses
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what routes are available for inoculation of embryonated eggs?
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yolk sac, chorioallantoic cavity, chorioallantoic membrane, amniotic sac
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what determines the route for inoculation of embryonated eggs?
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different virus, different affects
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how is a yolk sac innoculated?
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5-7d embryo, disinfect and drill hole, push 1" needle all the way in, seal with duco cement, incubate and look for death
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what are the advantages/disadvantages of yolk sac innoculation?
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easiest, but not commonly used because viruses generally grow in embry not the sac
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what viruses work well in yolk sac innoculations?
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avian encephalomyelitis virus, viral arthritis
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how is chorioallantoic cavity innoculated?
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9-11d embryo, drill hole at air space, incubate and look for death
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what are the advantages/disadvantages of chorioallantoic cavity innoculation?
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a little less easy but common
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what viruses work well in chorioallantoic cavity innoculation?
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newcastle disease virus, infectious bronchitis virus, avian influenza virus
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how is chorioallantoic membrane innoculated?
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10-12d, drill hole at air space for suction and 2nd hole at top for needle, incubate and look for membrane edema or focal necrosis
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what are the advantages/disadvantages of chorioallantoic cavity innoculation?
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quite difficult but common
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what viruses work well in chorioallantoic cavity innoculation?
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infectious laryngotracheitis virus, infectious bursal disease virus, fowl pox virus
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what are the advantages/disadvantages of amniotic sac innoculation?
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very difficult and rarely used
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how do we use an embryonated egg to identify viruses by in vitro assay?
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ie chicken with respiratory disease, obtain tracheal respiratory swab and soak in buffer, inject buffer into chorioallantoic cavity, wait for embryo death, harvest allantoic fluid (amplified virus), perform hemagglutination assay, identify specific virus
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