Study your flashcards anywhere!

Download the official Cram app for free >

  • Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off
Reading...
Front

How to study your flashcards.

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

A key: Read text to speech.a key

image

Play button

image

Play button

image

Progress

1/13

Click to flip

13 Cards in this Set

  • Front
  • Back
Can non-infectious virions in a virus sample contribute to CPE in tissue culture?
No
What is the advantage of using CPE?
CPE is the only method to directly count infectious units, or live infectious virions in a clinical/ laboratory specimen.
BUT IT IS NOT SPECIFIC-ONLY TELL YOU OF THE VIRAL PRESENCE IN THE CULTURE
What are plaques forming units (PFU)?
PFU are isolated zones of infection in the tissue culture.
These zones represent the cell-to-cell spread of virus from the originally infected cell at the heart of the plaque.
What does the agar medium do in the tissue culture forming PFU?
It prevents virions from moving around in the tissue culture by diffusion, but will allow the cell-to-cell spread of the virus infection.
How does tumor virus infection manifest in a tissue culture?
Abnormal proliferation of cells will result in vast piles or mountains of cells.
What is an inclusion body?
When virus infected cells are stained histologically the sites of viral replication or assembly can show up as an inclusion body.
The cellular location (nucleus or cytoplasm) may be characteristic of a particular virus.
What are some detection/ identification methods of viruses?
Virus cultures
Immunological methods (antibody against virion; may attach fluorescent abs)
PCR (identify viral nucleic acid)
Antibody tests (detect ab against a certain virus)
What are the most common ways of detecting the reaction of virons using specific antibody?
Neutralization
Complement fixation
Hemagglutination inhibition
Fluorescent ab
Radioimmunoassay, ELISA, etc.
Neutralization
The most specific way of conclusively identifying a virus along with PCR.
Specific ab is used to identify the virion.
Virions complexed with ab neutralized in their infectivity --> can't induce any PFU or CPE in tissue clutlre.
What is seroconversion?
The change in ab status from having no ab against a certain virus to having this ab.
Seropositive vs. seronegative
Paired acute vs. Convalescence
Before immune response vs. after immune response
Window period
Between paired acute and convalescent periods.
i.e. infected but not yet detectible ab against the specific ag.
What are some rapid diagnosis methods?
1) Viral protein atigen can be detected using fluorescent ab.
2) Viral nucleic acid can be identified by the PCR.