Virology 08: Hepatitis Ii

Front 1

HBV is aka ___. it's the main member of group ___

Back 1

post transfusion hep (PTH)
hepadnaviridae

Front 2

HBV virion components (4)

Back 2

DNA (mostly ds)
DNA pol
capsid proteins
envelope

Front 3

HBV capsid Ags (2)

Back 3

HBcAg
HBeAg

Front 4

envelope Ags

Back 4

HBsAg aka australia

Front 5

in addition to infective virions, called ___, HBV makes ___ (2). the purpose is ___

Back 5

Dane particle
filamentous
spherical
dilutes immune response

Front 6

X protein is a ___. it may be involved in ___

Back 6

transcription factor
carcinogenesis

Front 7

HBs and ___ are encoded in overlapping stretches of DNA

Back 7

polymerase

Front 8

HBV's large DNA strand has ___ sense. it is bound to ___

Back 8

minus
terminal protein

Front 9

HBV life cycle (9)

Back 9

1. virus enters cell by endocytosis
2. viral DNA enters nucleus
3. polymerase completes dsDNA
4. DNA goes to CCC DNA form
5. host RNA pol II makes pregenomic RNA and mRNA
6. pregnomic packaged into capsids
7. RT makes - stand off pregenomic RNA
8. DNA dep DNA pol makes + strand
9. DNA either goes back to nucleus to make a new CCC DNA or buds from host cell

Front 10

HBV polymerase functions (3)

Back 10

RT
RNAse H
DNA dependent DNA pol

Front 11

after the - strand of DNA is synthesized, the pregenomic RNA is degraded by ___

Back 11

RNAse H

Front 12

routes of HBV transmission (3)

Back 12

parenteral
sex
birth canal

Front 13

HBV incubation

Back 13

1-5 months

Front 14

HBV in children is frequently ___ but frequently causes ___

Back 14

asymptomatic
chronic infection

Front 15

HBV chronic infections are frequently ___ but can cause ___

Back 15

asymptomatic
HCC

Front 16

___ is the first marker for acute HBV infection. if there is recovery ___. in chronic infection, ___.

Back 16

HBsAg
it goes down
it stays high

Front 17

anti-HBs is present in ___ but not in ___
anti HBc IgM is present in ___, but not in ___. total anti-HBc is high in ___.

Back 17

acute
chronic
acute phase
chronic phase
both acute and chronic

Front 18

no HBs but high total anti-HBc means ___ but no ___. anti-HBc with anti-HBs means ___. anti-HBs without anti-HBc means ___

Back 18

previous infection and cure
immunity
cure with immunity
immunization

Front 19

HBV DNA in serum means

Back 19

active virus replication

Front 20

3 ways to identify HBV DNA

Back 20

dot blot hybridization
qualitative PCR
quantitative PCR

Front 21

in case of needle stick, do (3)

Back 21

1. check patient for HBS (and HIV)
2. if present, check Dr for anti-HBs
3. if not present, administer HB Ig (passive immunization)

Front 22

HDV is always associated with ___
its genome is ___, but it shares ___ with HBV

Back 22

HBV infection
ssRNA
HBsAg