Virology 04: Rna Virus Replication

Front 1

___ is a + sense ssRNA virus
it is part of the group ___, which is part of class ___

Back 1

polio
picornavirus
IV

Front 2

___ (2) is a - sense ssRNA virus
they are in class ___

Back 2

rabies
ebola
V

Front 3

___ is a group of segmented dsRNA viruses. they are in class ___

Back 3

reovirus
III

Front 4

___ is a group of segmented - sense ssRNA. they are in class ___

Back 4

orthomyxovirus
V

Front 5

class IV subfamilies

Back 5

picorna
flavi
calici

Front 6

picornavirus subtypes (4)

Back 6

enterovirus
rhinovirus
heparnavirus
aphtovirus

Front 7

___ is a hepatovirus

Back 7

HAV

Front 8

___ is an aphtovirus

Back 8

foot and mouth disease

Front 9

+ sense ssRNA viruses do not package ___ in the virion. instead, they ___.

Back 9

replication proteins
make them after infection

Front 10

polio genome undergoes ___ early in infection. it is covalently linked to ___ at ___. it has ___ to ensure ___

Back 10

direct translation
VPg
5' end
internal ribosome entry site (IRES)
cap-independent translation

Front 11

polio does host cell shut off by expressing ___. this operates by ___ (2)

Back 11

a multifunctional enzyme
enhances translation of mRNA with polio 5' UTR
cleaves CBP so host mRNA isn't translated

Front 12

in contrast to class IV, class V viruses need to ___

Back 12

package RNA-dependent RNA pol in the virion

Front 13

influenza virus genome has ___ segments

Back 13

8

Front 14

influenza envelope glycoproteins (2)

Back 14

hemagglutinin
neuraminidase

Front 15

HA binds to ___ on cell membrane. this does ___

Back 15

sialic acid
catalyzes fusion of envelope with membrane

Front 16

NA ___s in case of ___

Back 16

chops sialic acid off cell in to release viriion
failed penetration

Front 17

M1 is ___, M2 is ___

Back 17

matrix protein
ion channel

Front 18

M2 purpose

Back 18

allow proton flow ->
pH change ->
trigger fusion with endosome

Front 19

- sense RNA makes 2 kinds of transcripts: ___ and ___. the difference between the two is ___

Back 19

mRNA
antigenome
mRNA has cap stolen from cellular transcript and is not exactly complementary to genome; antigenome is exactly complementary and is used for viral replication

Front 20

antigenic drift is ___
antigenic shift is ___

Back 20

gradual change in antigens by mutation
large change in antigens by reassortment between 2 forms of the virus

Front 21

class VI is ___

Back 21

retroviruses

Front 22

although they are + sense RNA, retroviruses do not ___

Back 22

use genome as mRNA

Front 23

genes present in all retrovirus genomes (3)

Back 23

gag
pol
env

Front 24

each retrovirus virion has ___ copies of the genome complexed with ___

Back 24

2
reverse transcriptase

Front 25

gag codes for (4)

Back 25

group-specific Ag
capsid
matrix
nucleic acid binding proteins

Front 26

pol codes for (3)

Back 26

polymerase
protease
integrase

Front 27

env codes for (3)

Back 27

envelope
glycoproteins

Front 28

because of ___, mutations are frequent in HIV. This means ___ happens quickly.

Back 28

inaccuracy of RT
evolution of drug resistance

Front 29

enzymes packaged in HIV virion

Back 29

RT
integrase (IN)
protease (PR)

Front 30

HIV co-receptor on T cells is ___. on macrophages it's ___.

Back 30

CXCR4
CCR5

Front 31

reverse transcribed HIV dsDNA travels to nucleus as ____

Back 31

noncovalently-linked circle

Front 32

HIV uses a ___ to cause ___ slippage. This allows ___ to be translated ___% of the time, instead of ___%.

Back 32

suppression sequence
ribosome
pol
10
0

Front 33

___ viruses require cell lysis for release. ___ don't

Back 33

nonenveloped
enveloped

Front 34

in HSV, ___ binds to the cell surface to prevent superinfection

Back 34

gD