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30 Cards in this Set
- Front
- Back
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PRRS DX:
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CS, serology, ELISA, VN, IFA, immunoperoxidase monolayer (not differentiate carrier from vaccinates) histology, PCR & gene sequencing, VI, direct FA, in situ hybridization, RT- PCR
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EVA DX:
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Nested PCR to find carriers, VI RK13 and IF, sample nasal, blood, semen, placenta, serological test > 4 x
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Feline Calcivirus
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viral antigen in synovial macrophages, history, CS, ulcers on mouth, VI, PCR: oropharyngeal swabs
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RHD
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RT-PCR, western blot, negative staining, immunoelectron microscopy, immunostaining or ELISA, hemagglutination test (less sensitive), in situ hybridization (research), Ab in hemagglutination inhibition or ELISA
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VES
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Cs, VI, serology: ELISA, CFT, microscopy
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FMDV
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carrier animals: culture pharyngeal mucus, antigen detection, serology: ELISA, or CFT, RT-PCR to id carriers
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PEE
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serology: VN, CFT (use to differentiate strains), PCR, VI brain and spinal cord
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SVD
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VS, ELISA, direct CFT, VI, serology
humans - seroconversion |
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EEE
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serology, ELISA, plaque reduction neutralization assay, HI, CF
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WEE
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serology, VI
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WNV
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serology
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Japanese encephalitis
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CS, VI, rise in titer, neutralization, HI, IF, CF, ELISA: titer not best because of cross reactivity
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CSF
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lab test, VI, tissue sample, whole blood, ELISA, direct immunoflourescence, CS, Hx, serology ELISA, VN * comparative neutralization test
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BVD
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serology * VN, Cs, VI
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Border disease
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histological demonstration of pathognomic lesions in CNS with immunohistochemical staining of virus*, VI, viral antigen detection ELISA using heparinized EDTA, RT-PCR
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TGE
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CS: diarrhea, 100% mortality, ELISA, virus detect: mucus/feces, IEM, flourescent, immunoperoxidase, serology, VN, blocking ELISA using mAB
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PHED
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VI, hemagglutination, viral antigen: immunoflourescence, serology, VN, HI
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PED
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VI, rise in Ab
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FECV, FIPV
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polyclonal hypergammaglobulinemia in serum protein electrophoresis (IL6, stimulates B cells), IFA, ELISA, PCR*
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CVE
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electron microscopy, Rt- PCR, VN, ELISA, not grow well in tissue or cell culture system
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BCE
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hemadsorption: RBC adhere, initial: electron microscopy
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Rabies
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changes in CNS, Hx, ICIB, antigen demonstration- direct fluorescent Ab test & *immunoperoxidase of the medulla, cerebellum or hippocampus
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Vesicular stomatitis
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not as contagious as FMD, lesions, viral antigen, ELISA, CF, VN
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BEFV
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CS, paralysis, serology, immunoflourescences: less useful, PCR, BHK-21 vero cells used to propogate isolated virus
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RVF
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isolation, intracerebellar inoculation, VN, HI, CF, serology: PRNT*, ELISA-Ab or Ag detect
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Akabane
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deformed fetuses, stillborns, flexed or locked limbs, serology: adult Ab titer, VI/ID, immunoflourescence, SN, HI,CF, AGID< IF test
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NSD
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Vi, culture (BHK-21-c-13) immunoflourescence, AGID, cross rxn, sero tests, IFA, AGID, CF, indirect HA, ELISA, VN
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BTV
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CS, history, VI, ELISA, IFA, VN, serology, CF
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Orbivirus
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sampling, CS, Hx, VI/ID, serology, necropsy
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enteric disease
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feces/ intestinal content, immunoelectron microscopy, ELISA, SDS page to demonstrate RNA segments
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