Vibrio, Campy, Helicopter

Front 1

species of vibrio

Back 1

Vibrio cholerae

Vibrio parahemolyticus

Vibrio vulnificus

Front 2

what disease does vibrio cholerae cause?

how is it carried around

Back 2

cholera; it's a waterborne pathogen

Front 3

what does vibrio genus like? (environment)

Back 3

warm seawater

Front 4

shape of vibrio

Back 4

curved, like a COMMA

Front 5

What is the major pathogenic characteristic of V. cholerae?

-What 2 main symptoms result?

Back 5

Exotoxin, aka enterotoxin.
1. Diarrhea (rice water stools)
2. Severe dehydration

Front 6

symptoms of cholera

Back 6

diarrhea (rice water stools)

-severe dehydration

Front 7

What 4 features are used for diagnosing V. cholerae?

Back 7

1. Curved gnb
2. Growth/color on TCBS agar
3. Biochemical sugars
4. String reaction

Front 8

4 points included in lab diagnosis of V. cholerae:

Back 8

-if we know it's a curvy gnbacillus

-if it's hard to grow on normal enteric media, but grows well on TCBS.

-biochemical rxtns
-string reaction

Front 9

What is the purpose of TCBS agar?

Back 9

Allowing growth and differentiation of Vibrios; inhibiting enteric bugs.

Front 10

what sugars does v. cholera ferment?

Back 10

all of 'em: gluc, lact, and sucr

Front 11

what's the string reaction?

Back 11

when you mix vibrio cholerae with .5% sodium desoxycholate it makes a mucousy string. not very technical tho

Front 12

which vibrio is associated with shellfish/raw fish/seafood and in the far East?

Back 12

vibrio parahemolyticus

Front 13

what are the symptoms of vibrio parahemolyticus?

Back 13

Enteritis from shellfish; self-limiting, but severe sometimes bloody diahrrea.

Front 14

Which two vibrios are halophilic
How is this feature used to identify them?

Back 14

Parahemolytic and Vulnificus
-Makes them turn green-blue on TCBS agar.

Front 15

what does NaCl do to vibrio cholera?

Back 15

inhibits its growth.

Front 16

how many other species of vibrio are there? characteristics?

Back 16

9; nonpathogenic; oxidase positive GNB

Front 17

What disease is caused by Vibrio vulnificus:

Back 17

Septicemia
Wound/ear infections following exposure to seawater.

Boating accident...

Front 18

List 4 basic characteristics of Campylobacter:

Back 18

1. GNBacillus - CURVED like S
2. oxidase +
3. obligate microaerophile (blw srfc)
4. non-fermenter - no carbs

Front 19

what is the main pathogen of the campylobacters?

Back 19

campylobacter jejuni

Front 20

-How is C.jejuni transmitted?
-What disease/sx does it cause?

Back 20

-Via ingestine of uncooked poultry.

-ENTERITIS: diahrrea, fever, bloody stools

Front 21

2 Virulence factors produced by campylobacter jejuni:

Back 21

-enterotoxin
-cytotoxin

Front 22

selective media for campylobacter: contains:

Back 22

campy BA

brucella agar base + blood + antibiotics

inhibits normal flora from intestines

Front 23

what are the conditions necess for incubating campylobacter jejuni?

Back 23

42 degrees C
microaerophilic environment
capnophilic

Campy BA

Front 24

What 5 features of Campylobacter jejuni allow presumptive ID?

Back 24

Gram stain = gnb, curvy
Oxidase = positive
Catalase = positive
Motility = darting
Wet/moist colonies

Front 25

what bacteria is campylobacter jejuni very similar to?

Back 25

e. coli


both cause diahhrea
have similar biochemistries
treat w/ same drug

Front 26

what is campylobacter fetus ss. fetus?
what does it cause?

Back 26

a subspecies of campylobacter;
causes septicemia, is a very rare pathogen;

Front 27

what bug is know for causing gastritis and peptic ulcers?

Back 27

helicobacter pylori

Front 28

what does helicobacter pylori prolificly produce?

Back 28

urease; causes gastritis and peptic ulcers.

Front 29

what type of flagella does helicobacter have?

Back 29

lophotrichous

Front 30

3 specimens used to identify helicobacter pylori:

Back 30

-serum
-breath
-gastric mucosal biopsy

Front 31

what 3 key biochemical rxtns of helicobacter pylori allow rapid id?

Back 31

-urease
-catalase
-oxidase

-All three are POSITIVE

Front 32

what is helicobacter pylori treated with?

Back 32

ampicillin and bismuth salicylate

Front 33

Strategy of Helicobacter pylori I.D.:

Back 33

-endoscopy - biopsy specimen
-histology w/ special stains
-culture the bug
-urease test
-PCR to i.d. nucleic acid
-Serology to test for Ab in serum or whole blood.

Front 34

how does the urea breath test work? what organism is it for?

Back 34

helicobacter pylori.

the bug produces urease; give the host urea w/ labeled C; if the bug is there, it'll break down the urea and labeled CO2 will be exhaled.