Usmle Biochem Lab Techs.

Front 1

Procedure used to amplify a desired fragment of DNA

Back 1

Polymerase chain reaction (PCR)

Front 2

Electrophoresis of DNA

Back 2

Southern blot

Front 3

Electrophoresis of RNA

Back 3

Northern Blot

Front 4

Electrophoresis of protein

Back 4

Western blot

Front 5

Used to profile gene expression levels or to detect single nuvleotide polymorphisms (SNPs)

Back 5

Microarrays

Front 6

Used to determine wheather a particular antibody is present ie. anti-HIV, May test antibodies or antigens.

Back 6

Enzyme linked immunosorbent assay (ELISA)

Front 7

Used for specific localization of genes and direct visualization of anomalies (eg. microdeletions) at a molecular level.

Back 7

Flourescence in situ hibridization (FISH)

Front 8

Production of a recombinant DNA molecule that is self-perpetuating

Back 8

Cloning

Front 9

Dideoxynucleotides halt DNA polymerization at each base, generating sequences of various lenghts that encompass the entire original sequence. Terminated fragments are elctrophoresed and the original sequence can be deduced.

Back 9

Sanger DNA sequencing

Front 10

1. random insertion of gene into mouse genome (constitutive)
2. Targeted insertion or deletion of gene through homologous recombination with mouse gene.

Back 10

Model systems

Front 11

Used to diagnose chromosomal imbalances (autosomal trisomies, microdeletions, sex chromosome disorders) Can be performed on blood, bone marrow, amniotic fluid or placental tissue.

Back 11

Karyotyping