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20 Cards in this Set
- Front
- Back
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What is the test principle for glucose test strip?
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It is based on double sequential enzyme reaction.
One is glucose oxidase which catalyzes the formation of gluconic acid and H2O2. The other is peroxidase, which catalyzes the reaction of H2O2 with potassium iodidew chromogen to oxidize the chromogen. |
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Is the glucose test specific for glucose?
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Yes. Thereagent does not react with lactose, galactose, fructose or reducing metabolites of drugs, salicylates and nalidixic acid. App. 100mg/dl glucose in urine is detectable.
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What is the test principle for bilirubin?
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It is based on the coupling of bilirubin with a diazotized dichloroaniline.
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Is bilirubin normally detectable in urine?
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No. Even trace amounts usually warrant further investigation.
The test has a sensitivity 0f 0.4-0.8mg/dl. |
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What is the testprinciple for ketone?
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It is based on the reaction of acetoacetic acid with Na+ nitroprusside in a basic medium. This test does not react with beta-hydroxybutyric acid or acetone.
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What is the test principle for specific gravity?
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It is based on the apparant pKa change of certain pretreated polyelectrolytes in relation to the ionic concentration.
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What is the s.g. range on the strip test?
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1.000-1.030
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What is the test principle for blood?
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It is ased on the pseudoperoxidase action of hemoglobin and RBCs which catalyzes the reaction of,
3,3,5, 5' tetramethyl benzidine. |
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What is its sensitivity to blood?
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At time of manufacture, this test has asensitivity to free hemoglobin of 0.015mg/dl or 5-10 intact RBCs/ul urine. It is slightly more sensitive to free hemoglobin and myoglobin than to an intact erythrocyte.
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What is the test principle for pH?
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It is based on the double pH indicator method, where bromothymol blue and methyl red give distinguishable colors over the pH range of 5-9.
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What is the test principle for protein?
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It is ased on the protein error of indicator principle. At constant pH, the development of any green color is due to thew presence of protein.
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What is the protein sensitivity factor?
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The test area is ore sensitive to albumin than to globulin, hemoglobulin, Bence=Jones proteins and mucoprotein. It is sensitive to 15mg/dl albumin.
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What is the test principle for urobilinogen?
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It is based on a modified Ehrlich reaction in which p-diethylaminobenzaldehyde reacts with urobilinogen in an acid medium.
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What is the sensitivity of urobilinogen?
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It will detect U. in concentations as low as 0.2 EU/dl in urine.
In a healthy population, the normal urine U. range obtained with this test is 0.2-1.0. A result of 2.0EU/dl may be of clinical significance. |
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What is the test principle for nitrite?
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This test depends on the conversion of nitrate to nitrite by the action of Gram neg bacteria in the urine. The nitrite reacts with p-arsanilic acid to form a diazonium compound.
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Is nitrite normally found in urine?
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No. The nitrite area will be positive in a proportion of cases of significant infection, depending how long the urine specimens were retained in the bladder prior to collection.
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What is the test principle for leukocytes?
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It is based on the action of esterase present in leukocytes, which catalyzes the hydrolysis of an indoxyl ester derivative. This ester reats with a diazonium salt to produce a beige pink to purple color.
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How sensitive is the leukocyte test?
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It can detect as low as 10-15 WBC/ul. It will not react with RBCs or bacteria.
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What is the test principle for ascorbic acid?
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The composition comprises of a complex chelating agent with a polyvalent metal ion in its lower state to produce a color change.
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What is the sensitivity of ascorbic acid? Is it normally found in urine?
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It can detect ascorbic acid in concentrations as low as 10mg/dl in urine.
Yes. The output will vary according to the intake. The average urinary output ranges from 20-30mg/day. The reactivity of glucose test decreases as the sg and/or ascorbic acid of the urine increases. reactivity may vary with temperature as well. |
