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40 Cards in this Set
- Front
- Back
pH indicator |
Double indicator system reaction Methyl red 4-6 Bromthymol blue 6-9 |
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Protein indicator |
Protein error of indicators |
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Glucose indicator |
Double sequential enzyme reaction Glucose oxidase Peroxidase |
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Ketone indicator |
Sodium nitroprusside |
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Blood indicator |
Pseudoperoxidase |
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Bilirubin indicator |
Diazo reaction |
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Urobilinogen indicator |
Ehrlichs aldehyde reaction |
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Nitrite indicator |
Greiss reaction |
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Leuk esterase indicator |
LE catalyzes hydrolysis of acid esterase |
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What tests doe ascorbic acid affect |
False neg- glucose, blood, bili, nitrite, LE False pos- clinitest |
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Positive bili normal urobil |
Bile duct obstruction |
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Pos or neg bili pos urobil |
Liver damage |
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Neg bili very pos urobil |
Hemolytic disease |
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Significance of pH |
Respiratory/metabolic ketoacidosis Renal calculi Defects in nephron tubule Determination of old urine |
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Renal calculi |
Acidic conditions |
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Significance of protein |
Proteinuria seen in early renal disease Only detects albumin <10 mg/dl is neg |
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Significance of glucose |
Screening for DM Glucose in urine may be incs in blood Pancreas disorders (insulin) Hyperactive hormone disorders |
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Renal threshold of glucose |
160-180 mg/dl |
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Significance of ketone |
Formed by fat breakdown Starvation, vomiting, diabetic acidosis |
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Significance of blood |
Hematuria Hemoglobinuria Myoglobinuria |
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Hemoglobinuria |
Hemolytic anemia Transfusion reaction |
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Myoglobinuria |
Trauma Prolonged coma Muscle wasting disease |
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Significance of bilirubin and urobilinogen |
Liver disease Bile duct obstruction Hemolytic disease |
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Significance of nitrites |
Gram neg rods Reduce nitrate to nitrites Screening for UTI |
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Significance of leukoesterase |
Presence of granulocytes and monocytes UTI or inflammation |
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Pyuria |
WBC in urine |
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Unconjugated bili |
Not water soluble Initial state of bili |
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Route of bili |
Unconjugated Albumin attaches brings to liver Conjugated in liver (safer) Travels to intestines bacteria convert to urobilinogen excreted into feces |
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Type of bili seen in urine |
Conjugated Unconjugated (attached to albumin) is too big to be filtered into urine |
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Why would conjugated bili be seen in urine |
If it can't exit through the duct into intestines it will back up into plasma and since it is water soluble it will be excreted in urine |
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Dipstick protein color |
Yellow to blue |
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Dipstick glucose color |
Green to brown |
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Dipstick ketone detects what |
Acetoacetic acid |
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3 ketone bodies |
Acetoacetic acid Acetone Beta hydroxybuterate |
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Interfere with ketone test |
False decr- old urine (bacteria use up)
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False pos for blood |
Menstrual blood, oxidizing agent, peroxide cleaners |
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False neg for blood |
Ascorbic acid, high SG/crenated cells Won't lyse so they look neg |
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Ictotest |
Confirmatory for bili Washes away interfering substances |
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False neg for bacteria |
Not enough time for nitrate to go to nitrite Not all bacteria reduce (GPC & yeast) No urinary nitrate Ascorbic acid Too many bacteria turning nitrite to nitrogen gas |
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Primary cause of false neg for glucose |
Old specimen - glycolysis |