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40 Cards in this Set

  • Front
  • Back

pH indicator

Double indicator system reaction


Methyl red 4-6


Bromthymol blue 6-9

Protein indicator

Protein error of indicators

Glucose indicator

Double sequential enzyme reaction


Glucose oxidase


Peroxidase

Ketone indicator

Sodium nitroprusside

Blood indicator

Pseudoperoxidase

Bilirubin indicator

Diazo reaction

Urobilinogen indicator

Ehrlichs aldehyde reaction

Nitrite indicator

Greiss reaction

Leuk esterase indicator

LE catalyzes hydrolysis of acid esterase

What tests doe ascorbic acid affect

False neg- glucose, blood, bili, nitrite, LE


False pos- clinitest

Positive bili normal urobil

Bile duct obstruction

Pos or neg bili pos urobil

Liver damage

Neg bili very pos urobil

Hemolytic disease

Significance of pH

Respiratory/metabolic ketoacidosis


Renal calculi


Defects in nephron tubule


Determination of old urine

Renal calculi

Acidic conditions

Significance of protein

Proteinuria seen in early renal disease


Only detects albumin


<10 mg/dl is neg

Significance of glucose

Screening for DM


Glucose in urine may be incs in blood


Pancreas disorders (insulin)


Hyperactive hormone disorders

Renal threshold of glucose

160-180 mg/dl

Significance of ketone

Formed by fat breakdown


Starvation, vomiting, diabetic acidosis

Significance of blood

Hematuria


Hemoglobinuria


Myoglobinuria

Hemoglobinuria

Hemolytic anemia


Transfusion reaction

Myoglobinuria

Trauma


Prolonged coma


Muscle wasting disease

Significance of bilirubin and urobilinogen

Liver disease


Bile duct obstruction


Hemolytic disease

Significance of nitrites

Gram neg rods


Reduce nitrate to nitrites


Screening for UTI

Significance of leukoesterase

Presence of granulocytes and monocytes


UTI or inflammation

Pyuria

WBC in urine

Unconjugated bili

Not water soluble


Initial state of bili

Route of bili

Unconjugated


Albumin attaches brings to liver


Conjugated in liver (safer)


Travels to intestines bacteria convert to urobilinogen excreted into feces

Type of bili seen in urine

Conjugated


Unconjugated (attached to albumin) is too big to be filtered into urine

Why would conjugated bili be seen in urine

If it can't exit through the duct into intestines it will back up into plasma and since it is water soluble it will be excreted in urine

Dipstick protein color

Yellow to blue

Dipstick glucose color

Green to brown

Dipstick ketone detects what

Acetoacetic acid

3 ketone bodies

Acetoacetic acid


Acetone


Beta hydroxybuterate

Interfere with ketone test

False decr- old urine (bacteria use up)


False pos for blood

Menstrual blood, oxidizing agent, peroxide cleaners

False neg for blood

Ascorbic acid, high SG/crenated cells


Won't lyse so they look neg

Ictotest

Confirmatory for bili


Washes away interfering substances

False neg for bacteria

Not enough time for nitrate to go to nitrite


Not all bacteria reduce (GPC & yeast)


No urinary nitrate


Ascorbic acid


Too many bacteria turning nitrite to nitrogen gas

Primary cause of false neg for glucose

Old specimen - glycolysis