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10 Cards in this Set
- Front
- Back
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Urea is the product of... |
-decarboxylation of certain of certain amino acid -hydrolyzed to ammonia and carbon dioxide by bacteria containing urease |
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Enteric bacteria that metabolize urea |
-Porteus -Morganella -Providencia (rapid urease positive organisms) |
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Urea Agar was formulated to... |
-differentiate rapid urease positive bacteria from slower urease positive and urease negative bacteria |
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Urea Agar contains |
-urea -peptone -potassium phosphate -glucose -phenol red (indicator) |
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Peptone and glucose |
provide essential nutrients for a broad range of bacteria |
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Potassium phosphate |
mild buffer used to resist alkalinization of the medium from peptone metabolism |
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Phenol red |
yellow or orange below pH 8.4 and red or pink above |
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Change to Pink |
-urea hydrolysis to ammonia by urease positive organisms will overcome the buffer in the medium and change it from orange to pink -rapid urea hydrolysis and strong urease production will turn the entire slant pink within 24 hours |
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Orange or yellow |
No urea hydrolysis organism does not produce urease or cannot live in broth |
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1. + urea rapid 2. Control 3. Negative |
