U4 Cell Signalling

Front 1

Types of Cell to cell signalling

Back 1

Hint 1

Front 2

Hydrophilic and hydrophobic mol

Back 2

Front 3

Signaling of acetylcholine

Back 3

One signal molecule can perform numerical function

1. In heart pacemaker sale at decrease rate of_____

2 it salivary gland cell it causes secretion

3 in skeletal muscle cell at causes contraction

Front 4

Major types of cell signaling receptor

Back 4

1.Ion channel coupled receptor

2.G protein coupled receptor

3.enzyme coupled receptor

Front 5

Cell signaling by phosphorylation and GTP binding

Back 5

Front 6

Regulation of Monomeric GTPase enzyme

Back 6

Hint 6

G proteins belong to the larger group of enzymes called GTPases,

that bind to the nucleotide guanosine triphosphate and hydrolyze it to guanosine diphosphate.

Front 7

How does upstream signal effects gene expression

Back 7

Hint 7

Upstream signals are those signal which comes from outside

Front 8

What is scaffold protein and how does it work

Back 8

Scaffold proteins are adaptor like protein

A ligand would bind to a receptor and it will activate intracellular signaling proteins( by auto posphorylation).

Front 9

Write a short note on G-protein couple receptor

Back 9

Hint 9

Front 10

Note on sub units of g protein. Name Effector of protein

Back 10

Front 11

does affector activate g protein or does g protein activate affector.

Back 11

Adenyl cyclase is example of effector enzyme and it is activated by g protein G-a subunit

Front 12

describe the sub units of g protein and The working and reaction that occurs in g protein when the receptor is activated

(What is Gs and AH domain)

Back 12

Hint 12

Front 13

How is cAMP formed and degraded. Which enzyme does catalysis and hydrolysis of cAMP

Back 13

Front 14

Give few examples of hormone their target tissue and major responses by them.

Back 14

Front 15

Activation of pka and its function

Back 15

Hint 15

Cyclic amp dependent protein kinase

Front 16

Terms

a.Effector molecule ,

b.2° messenger

c. Upstream signal

d.Scaffold protein

Back 16

an effector molecule is usually a small molecule that selectively binds to a protein and regulates its biological activity

2° messenger is an intracellular molecule the changes in concentration in response to environmental signal and involve when conveying info inside the cell.

Upstream signal bahar se aaya Hua signal hai.

Scaffold protein is also known as adaptor protein

Scaffold has many binding sites which when not phosphorylated is known as inactive intracellular signalling protein

Front 17

What is Autocrossphosphorylation

Back 17

In Autocrossphosphorylation- from protein dimer Pov the dimer is phosphorylating itself but from the monomer pov monomers are phosphorylating each other(hence Cross word );Ex rtk

It is also known as trans-auto-phosphorylation

Pov- point of you

Front 18

What is domain

Back 18

It is a region of protein's polypeptide chain that is cell stabilizing and that fold independently from the rest. It is compact 3D structure they often form functional unit. It often form functional unit

(protein polypeptide ki voh region jo ki khud se fold kar leta hai aur khud ko stable kar leta hai. Aur usko Baki polypeptide ke region se Koi Lena Dena nahin Hai.)

Front 19

Activation curve of allosteric protein

Back 19

X axis is effector molecule and y axis is sensitivity

(In "A" thode se Hi effector molecule mein vohhighly sensitive tha

.in D itne sare effector molecule hone ke bad halka sa sensitivity show ki usne D ne)

Front 20

Coincidence signal detector

Back 20

Front 21

Gpcr 1° AC pathway

Back 21

Hint 21

CRE- cAMP response element

CREB - c a m p response element binding protein

Cbp - c r e b binding protein.

Front 22

Draw table of some member of g protein family and write the function.

Back 22

Front 23

Gpcr plc B pathway

Back 23

Hint 23

Front 24

Step wise activation of cam kinase II pathway

Back 24

Hint 24

Front 25

Component of rhodopsin

Back 25

Front 26

Activation of rhodopsin molecule

Back 26

Hint 26

Front 27

What is PDE in rhodopsin cell signalling

Back 27

Pde is cyclic GMP phosphodiesterase. It Breaks cGMP( cyclic) into GMP(linear).

Front 28

NO cell signaling breif . three types of nos enzyme, where are they found. And which is the only free radical beneficial to human

Back 28

Hint 28

Front 29

In brief draw all the five cell signal in pathway in one picture ( master slide )

Back 29

Front 30

Glycogen pathway .( Which type of liver cell produce which hormone and its effects)

Back 30

Revise form L4 in beganing

(Mnemonic AG bids)

Hint 30

Front 31

What are the gpcr regulation pathways and describe densitization

Back 31

Hint 31

Front 32

What signaling molecule causes glucose storage and mobilization.

write a short flow chart summary of all the increasing and decreasing level of molecule example camp, glucagon with respect to each other(glucagon signaling pathway)

Back 32

Glycogen phosphorylase is activated in the response to glucagon and ephinephrin. Where as glycogen synthase is activated in response to insulin.

Hint 32

Front 33

Which molecules get active while phosphorylation or dephosphorylation in glucagon pathway

Back 33

Front 34

Summary of G- protein association and association on getting signal

Back 34

Front 35

Read lecture 3 of unit 4 from PPT

Back 35

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