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27 Cards in this Set
- Front
- Back
What genus are part of Proteeae
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Proteus, Morganella, Providencia
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What test are used to differentiate between Proteus, Morganella, and Providenicia
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H2S +. Swarm +, D- man (manose) -, Gel liq (gelatinase) +, Lipase +
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Proteeae produce_____ and are positive for_____ which allows them to show _____ infection
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phenylalanine deaminase
urease Urinary Tract |
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what test are used to differentiate between P. mirabilis and other Proteus?
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Ornithine +
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What test are used to differentiate P. hauseri/P. vulgaris from other proteus? Differentiate between P. hauseri and P. vulgaris
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Indole + for both
Salicin + for P. vulgaris |
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What inhibits swarming?
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Phenyl ethyl alcohol
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In KIA proteus has _____ slant and ______ butt.
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Alkaline - no lactose fermentation
Acid - glucose fermentation |
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What test can be used to differentiate morganella from proteus?
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Swarm -
H2S - |
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what tests are used to identify providencia
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Fermentation of inositol, adonitol, arabitol, trehalose, and galactose
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How do Y. pestis get transmitted from Fleas to Humans?
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Y. pestis clogs the flea proventriculus (gut)
Less than 27 C Y. pestis produces coagulase Flea cannot get food, regurgitates Y. pestis in humans |
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_____ and ______ are stains for Y. pestis
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Gram Stain
Wayson's Stain (methylene blue, carbofuschin) |
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On blood agar plat Y. Pestis exhibits ____ hemolysis
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Gamma
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infection 7 days after flea bite
sudden onset of fever chills weakness headache Painful buboes |
Bubonic PLague
75% mortality if untreated |
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Droplet nuclei
short incubation 2-3 days fever, maliase, pulmonary signs cough and chest pain Sepsis and Death |
Pneumoni PLague
90% mortality rate |
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Direct infection by flea bite in blood stream
High fever delirium seizures septic shock disseminated intravascular Black hemorrhaegic splotches |
Septicemic Plague
black death no bubous |
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______,______,_____, and ________ are possible treatments for Y. pestis
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streptomycin, gentamicin, tetracycline, and chloramphenicol
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Three important things about Y. pestis
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1. 1st time iron played a pathogenic role
2. Discovery of type III secretion system 3. Role of flea in disease |
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Virulence factors of three strains
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YadA
LcrV Adhesins/Invasins |
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____ binds intestinal mucin and collagen. Provides resitance to ______.
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YadA (yersinia adhesin A)
phagocytosis |
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LcrV
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secreted extracellularly opens channels in euk membranes
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_____, ____, and _____ are mans of iron acquistion
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Siderophores, Heme Utilization, Hemin Storage
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Siderophores
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Yersinia Bactin
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Hemin Storage
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bind hemin on the surface, gives colonies dark brown color
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What two test differentiate Y. pestis from pseudotuberculosis and enterocolitica
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Motility at 22 C -
Urease - |
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Wha two test diffrentiate Y. enterocolitica from pseudotuberculosis and pestis
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Sucrose ferm +
Orn decarb + |
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Tribe Erwinieae
genus Erwinia species amylovora |
fire blight
plugs xylem treateed by depolymerizin phage and bacteriocin |
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E. herbiola
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frost damage in trees
ice nucleation at -1 C |