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34 Cards in this Set
- Front
- Back
Tribe Salmonelleae
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contains one single genus (Salmonella)
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What does the Vi antigen in salmonella do?
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Covers up the O antigens, and LPS similar to a capsule
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what KIA result does Salmonella have?
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Alkaline slant/Acid Butt, with Gas productoin and H2S production
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Are salmonella butanediol fermenters
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No, negative reaction on VP
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Are salmonella mixed acid fermenters
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YEs, positive reaction on methly red
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True/False
salmonella have the ability to hydrolyze urea |
False
slamonella test negative for urease |
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Can salmonella decarboxylate Lysine, Arginine, and Ornithine
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Positive decarboxylation for Lys and Orn.
Variable results for arginine |
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What mode of infection does salmonellosis have?
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Oral-Fecal route. consuming water, food, or milk contaminated by human of animal fecal
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What is the reservoir fo S. typhi
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Humans only.
Carrier state - can shed organisms up to a year after remission of symptoms |
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How can we control Salmonella on eggs
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Spray outside of the eggs with chlorine, hydrogen peroxide
or vaccinate chickens |
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Types of salmonella infections
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Gastroenteritis, BActeremia (Septicemia), Enteric fever
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Disease process of Salmonellosis
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survive acid of the stomach, move through the intestinal mucosal layer, attach to the M cells and ruffle, multiply in macrophages and shut down the oxidative response
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Symptoms of enterocolitis
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Penetration of the mucosal cells, results in inflammation and diarrhea, headaches, chills, abdominal pains, nausea
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What is the treatment of enterocolitis
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No treatment, fluids and electrolytes replacement
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What is a type of Enteric fever and what organism cuases this
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Typhoid fever
S.typhi |
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How does S. typhi cause Typhoid fever
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Cells enter draining lymph nodes where the bacteria multiply and spread to phagocytes of the liver, gall bladder, and spleen
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symptoms of Typhoid fever
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7-14 day incubation, maliase, loss of appetite, headache, fever, abdominal rose spots
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Where can S. typhi be found in infected individuals
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Bimodal infection
first two weeks found in the blood, not the stoll Second part not found in the blood, found in stool |
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What are good tests to ISOLATE Salmonella?
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MacConkey agar - Lac - , colorless colonies
Hextoen Agar - H2S production + |
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What are symptoms of septicemia
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Spiking fever, lesions in bile duct, kidneys, spleen, and lungs
Found in blood |
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What is Widal reaction? What is it used to diagnose?
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USed for Serodiagnosis, used to diagnose Typhoid Fever when no isolates can be found
Measure agglutination host antibodies to "O" and "H" antigens |
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What disease can Citrobacter koseri cause
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children - meningitis
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Is Tribe Citrobactereae a butanediol fermenter? Mixed Acid Fermenter?
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VP -
MR + |
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What test can differentiate Tribe Klebsiella from Tribe salmonella
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KIA
Salm - Alk/Acid with H2S Kleb - Acid/Acid with no H2S |
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What test is a good differentiator for Klebsiella oxtoca and Klebsiella pneumoniae ssp. pneumonia, ozonia, rhinoscleromatis
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Indole test
K. oxytoca - Indole + K. pneumoniae ssp, Indole - |
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What does K. pneumoniae cause
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Pneumonia with necrosis and brick red sputum.
Also enteritis, UTI, meningitis, and septicemia |
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what is so dangerous about Klebsiella (genus)
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HArbor many antimicrobial resistance plasmids
Some can grow on carbenicillin/ampicillin |
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Genus Enterobacter
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Motile rods, MR - , VP +, H2S -
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What disease can Enterobacter cloacae cause
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HUs-like syndrome in infants with toxins
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what are the best tests to differentiate between E. aerogenes/E. cloacae
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E. aerogenes - Lys +, Arg -
E. cloacae - Lys -, Arg + |
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Genus Pantoea
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P. agglomerans, a biopesticide
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What organism from Tribe Klebsielleae makes the scent of human feces, Skatole
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Hafnia alvei
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What is the only enteric genus that produces DNase
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Serratia
G - Lipase, gelatinase, DNase + |
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Serratia marcescens is often associated with what types of infections and what is the red pigment it produces when cultured? What temp?
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The human infections; pneumonia and septicemia
The red pigment is prodigiosin and is only produced at 25 degrees celsius |