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34 Cards in this Set

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Tribe Salmonelleae
contains one single genus (Salmonella)
What does the Vi antigen in salmonella do?
Covers up the O antigens, and LPS similar to a capsule
what KIA result does Salmonella have?
Alkaline slant/Acid Butt, with Gas productoin and H2S production
Are salmonella butanediol fermenters
No, negative reaction on VP
Are salmonella mixed acid fermenters
YEs, positive reaction on methly red
True/False
salmonella have the ability to hydrolyze urea
False
slamonella test negative for urease
Can salmonella decarboxylate Lysine, Arginine, and Ornithine
Positive decarboxylation for Lys and Orn.
Variable results for arginine
What mode of infection does salmonellosis have?
Oral-Fecal route. consuming water, food, or milk contaminated by human of animal fecal
What is the reservoir fo S. typhi
Humans only.

Carrier state - can shed organisms up to a year after remission of symptoms
How can we control Salmonella on eggs
Spray outside of the eggs with chlorine, hydrogen peroxide
or vaccinate chickens
Types of salmonella infections
Gastroenteritis, BActeremia (Septicemia), Enteric fever
Disease process of Salmonellosis
survive acid of the stomach, move through the intestinal mucosal layer, attach to the M cells and ruffle, multiply in macrophages and shut down the oxidative response
Symptoms of enterocolitis
Penetration of the mucosal cells, results in inflammation and diarrhea, headaches, chills, abdominal pains, nausea
What is the treatment of enterocolitis
No treatment, fluids and electrolytes replacement
What is a type of Enteric fever and what organism cuases this
Typhoid fever
S.typhi
How does S. typhi cause Typhoid fever
Cells enter draining lymph nodes where the bacteria multiply and spread to phagocytes of the liver, gall bladder, and spleen
symptoms of Typhoid fever
7-14 day incubation, maliase, loss of appetite, headache, fever, abdominal rose spots
Where can S. typhi be found in infected individuals
Bimodal infection
first two weeks found in the blood, not the stoll
Second part not found in the blood, found in stool
What are good tests to ISOLATE Salmonella?
MacConkey agar - Lac - , colorless colonies
Hextoen Agar - H2S production +
What are symptoms of septicemia
Spiking fever, lesions in bile duct, kidneys, spleen, and lungs

Found in blood
What is Widal reaction? What is it used to diagnose?
USed for Serodiagnosis, used to diagnose Typhoid Fever when no isolates can be found

Measure agglutination host antibodies to "O" and "H" antigens
What disease can Citrobacter koseri cause
children - meningitis
Is Tribe Citrobactereae a butanediol fermenter? Mixed Acid Fermenter?
VP -
MR +
What test can differentiate Tribe Klebsiella from Tribe salmonella
KIA
Salm - Alk/Acid with H2S
Kleb - Acid/Acid with no H2S
What test is a good differentiator for Klebsiella oxtoca and Klebsiella pneumoniae ssp. pneumonia, ozonia, rhinoscleromatis
Indole test
K. oxytoca - Indole +
K. pneumoniae ssp, Indole -
What does K. pneumoniae cause
Pneumonia with necrosis and brick red sputum.
Also enteritis, UTI, meningitis, and septicemia
what is so dangerous about Klebsiella (genus)
HArbor many antimicrobial resistance plasmids

Some can grow on carbenicillin/ampicillin
Genus Enterobacter
Motile rods, MR - , VP +, H2S -
What disease can Enterobacter cloacae cause
HUs-like syndrome in infants with toxins
what are the best tests to differentiate between E. aerogenes/E. cloacae
E. aerogenes - Lys +, Arg -
E. cloacae - Lys -, Arg +
Genus Pantoea
P. agglomerans, a biopesticide
What organism from Tribe Klebsielleae makes the scent of human feces, Skatole
Hafnia alvei
What is the only enteric genus that produces DNase
Serratia
G -
Lipase, gelatinase, DNase +
Serratia marcescens is often associated with what types of infections and what is the red pigment it produces when cultured? What temp?
The human infections; pneumonia and septicemia
The red pigment is prodigiosin and is only produced at 25 degrees celsius