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48 Cards in this Set

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What genus makes up tribe Escherichieae
Escherichia and Shigella
What three tests are good for differentiation of Escherichia and Shigella?
Motility: E +, S-
Lysine Decarbox: E+, S-
Gas from Glucose: E+, S-
What are colicins?
Cell free bacteriocins that inhibit other bacteria.
20 molecules
A-V
What mode of actions does colicin E2 have?
Inhibits DNA synthesis
what other modes are modes of activity do colicins have?
membrane depolarization, DNA endonuclease, ribosome inactivation
Genus Escherichia: gram stain/morphology
Gram - Straight Rods
Genus Escherichia: Oxidase =/-
Negative
Genus Escherichia: Citrate Utilization
Negative
Genus Escherichia: Production of H2S
negative
Genus Escherichia: Indole production
positive
Genus Escherichia: Butanediol fermentation
negative
Genus Escherichia: Urease production
Negative
Genus Escherichia: Lysine/Ornithine decarb
Positive
Genus Escherichia: KIA results
Glucose/LActose fermentation, no H2S production

Yellow (A) Slant and Butt
What do type 1 fimbriae (pili) allow in E. Coli?
binding to urinary slime
What does E. Coli K1 cause?
2nd leading cause of neonatal meningitis
What does E. Coli O55 and O111 cause?
Neonatal Diarrhea
Two mechanisms of diarrhea
1. Invasion and destruction of mucosal epithelial cells with subsequent loss of reabsorptive function

2. Production of an exotoxin that causes fluid secretion from epithelium cells
Method of intestinal infection in E. Coli
Attachment by pili to ileum and jejunum

enterotoxins
Two types of enterotoxin in E. Coli
LT and ST
LT
high molecular weight, heat labile.

Acts by catalyzing the addition of AADP-ribose that stimulates cyclase

Results in increased cAMP production, and oupouring of fluid, K and Cl
ST
low molecular weight, heat stable

Stimulates production of guanylate cyclase
ETEC causes what disease and by what mechanism
"Traveler's Diarrhea"
Plasmid encoded Enterotxins, LT and ST, do not damage mucosal epithelium
Who is affected by traveler's diarrhea
All ages, transmission by fecal-oral route, requires 10^6-10^9 organisms
which e.Coli strains are ETEC
06, 08, 015, 020
What is Traveler's Diarrhea called in infants
Cholera infantum
What disease is caused by EPEC
infantile diarrhea
What is the mode of EPEC pathogenesis
Attachment to intestinal epithelial cells and cause effacing lesions.

Noscomial acquired
Symptoms of Traveler's Diarrhea
profuse watery diarrhea, mild abdominal cramps, vomiting
Symptoms of infantile diarrhea
low grade fever, malaise, vomiting,
diarrhea, large amount of mucous, no gross blood
What serogroups cause EPEC
055. 086, 0111, 0119
What disease is caused by EIEC
Dysentery like diarrhea
what mechanisms do EIEC invade wtih
EIEC invade epithelial cells
What are the symptoms of EIEC
dysentery, fever, colitis, urgency and painful straining at stools, blood, mucous, and WBCs in the stool
What strains of E. Coli cause EIEC
E. coli 028ac, 029, 0112ac, 0124
What disease does EHEC cause
hemolytic-uremic syndrome
What are symptoms of EHEC infection
bloody diarrhea, no WBCs, no fever, abdominal pain, may progress to HUS
Virulence factors of EHEC
adhesion similar to EPEC
Shiga Toxin 1 and 2 receptors in kidney and intesinal cell walls
- acute kidney failure
(Lysogenic Conversion from phage)
Important E. coli in EHEC
E. coli 0157:H7
Symptoms of E. Coli 0157:H7
hemorrhagic colitis, abdominal cramps, red cell lysis, kidney failure
157th O antigen, 7th H antigen
Virulence mechanism Cytotoxin
What test is good for detection of E. coli 0157:H7? Results?
sorbitol MacConkey agar in the acute phase of illness

sorbitol -
Treatment of E. coli 0157:H7
fluoroquinolones trimethoprim
What symptoms are caused by EAEC
watery diarrhea, low grade fever, vomiting, dehydration

Virulence factor adherence and ST-like toxin
What is a good test to differentiate E. hermanii from E. coli and E. fergusonii
lysine decarb
E.coli +
E.fergusonii +
E. hermanii -
Treatment of UTI caused by E. coli
sulfonamides and ampicillin
Treatment of Sepsis caused by E. coli
gentamicin
Treatment of Neonatal meningitis caused by E. coli
gentamicin and ampicillin
Treatment of diarrheal diseases caused by E. coli
no antiobiotics, trimethoprim-sulfamethoxazole may shorten the length of disease