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48 Cards in this Set
- Front
- Back
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What genus makes up tribe Escherichieae
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Escherichia and Shigella
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What three tests are good for differentiation of Escherichia and Shigella?
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Motility: E +, S-
Lysine Decarbox: E+, S- Gas from Glucose: E+, S- |
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What are colicins?
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Cell free bacteriocins that inhibit other bacteria.
20 molecules A-V |
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What mode of actions does colicin E2 have?
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Inhibits DNA synthesis
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what other modes are modes of activity do colicins have?
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membrane depolarization, DNA endonuclease, ribosome inactivation
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Genus Escherichia: gram stain/morphology
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Gram - Straight Rods
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Genus Escherichia: Oxidase =/-
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Negative
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Genus Escherichia: Citrate Utilization
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Negative
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Genus Escherichia: Production of H2S
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negative
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Genus Escherichia: Indole production
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positive
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Genus Escherichia: Butanediol fermentation
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negative
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Genus Escherichia: Urease production
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Negative
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Genus Escherichia: Lysine/Ornithine decarb
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Positive
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Genus Escherichia: KIA results
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Glucose/LActose fermentation, no H2S production
Yellow (A) Slant and Butt |
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What do type 1 fimbriae (pili) allow in E. Coli?
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binding to urinary slime
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What does E. Coli K1 cause?
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2nd leading cause of neonatal meningitis
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What does E. Coli O55 and O111 cause?
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Neonatal Diarrhea
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Two mechanisms of diarrhea
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1. Invasion and destruction of mucosal epithelial cells with subsequent loss of reabsorptive function
2. Production of an exotoxin that causes fluid secretion from epithelium cells |
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Method of intestinal infection in E. Coli
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Attachment by pili to ileum and jejunum
enterotoxins |
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Two types of enterotoxin in E. Coli
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LT and ST
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LT
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high molecular weight, heat labile.
Acts by catalyzing the addition of AADP-ribose that stimulates cyclase Results in increased cAMP production, and oupouring of fluid, K and Cl |
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ST
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low molecular weight, heat stable
Stimulates production of guanylate cyclase |
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ETEC causes what disease and by what mechanism
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"Traveler's Diarrhea"
Plasmid encoded Enterotxins, LT and ST, do not damage mucosal epithelium |
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Who is affected by traveler's diarrhea
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All ages, transmission by fecal-oral route, requires 10^6-10^9 organisms
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which e.Coli strains are ETEC
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06, 08, 015, 020
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What is Traveler's Diarrhea called in infants
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Cholera infantum
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What disease is caused by EPEC
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infantile diarrhea
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What is the mode of EPEC pathogenesis
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Attachment to intestinal epithelial cells and cause effacing lesions.
Noscomial acquired |
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Symptoms of Traveler's Diarrhea
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profuse watery diarrhea, mild abdominal cramps, vomiting
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Symptoms of infantile diarrhea
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low grade fever, malaise, vomiting,
diarrhea, large amount of mucous, no gross blood |
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What serogroups cause EPEC
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055. 086, 0111, 0119
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What disease is caused by EIEC
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Dysentery like diarrhea
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what mechanisms do EIEC invade wtih
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EIEC invade epithelial cells
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What are the symptoms of EIEC
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dysentery, fever, colitis, urgency and painful straining at stools, blood, mucous, and WBCs in the stool
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What strains of E. Coli cause EIEC
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E. coli 028ac, 029, 0112ac, 0124
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What disease does EHEC cause
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hemolytic-uremic syndrome
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What are symptoms of EHEC infection
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bloody diarrhea, no WBCs, no fever, abdominal pain, may progress to HUS
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Virulence factors of EHEC
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adhesion similar to EPEC
Shiga Toxin 1 and 2 receptors in kidney and intesinal cell walls - acute kidney failure (Lysogenic Conversion from phage) |
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Important E. coli in EHEC
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E. coli 0157:H7
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Symptoms of E. Coli 0157:H7
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hemorrhagic colitis, abdominal cramps, red cell lysis, kidney failure
157th O antigen, 7th H antigen Virulence mechanism Cytotoxin |
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What test is good for detection of E. coli 0157:H7? Results?
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sorbitol MacConkey agar in the acute phase of illness
sorbitol - |
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Treatment of E. coli 0157:H7
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fluoroquinolones trimethoprim
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What symptoms are caused by EAEC
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watery diarrhea, low grade fever, vomiting, dehydration
Virulence factor adherence and ST-like toxin |
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What is a good test to differentiate E. hermanii from E. coli and E. fergusonii
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lysine decarb
E.coli + E.fergusonii + E. hermanii - |
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Treatment of UTI caused by E. coli
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sulfonamides and ampicillin
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Treatment of Sepsis caused by E. coli
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gentamicin
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Treatment of Neonatal meningitis caused by E. coli
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gentamicin and ampicillin
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Treatment of diarrheal diseases caused by E. coli
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no antiobiotics, trimethoprim-sulfamethoxazole may shorten the length of disease
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