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20 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Three types of transport proteins?
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Channels, Transporters, ATPase pumps
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Channels
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single file flow, continuous but specific & often gated
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Transporters
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one factor at a time via conformational change of protein. rate of diffuse slower than channel
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ATPase pumps
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ACTIVE transport via direct hydrolysis of ATP. (against conc. gradi)
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Three types of Transporters
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Uniporter, Symporter, Antiporter
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Uniporter
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one factor across membrane
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Symporter
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simultaneously 2 in same direction
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Antiporter
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simultaneously transports two in diff directions
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P class pumps
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tetrameres, two regulatory & two passage; ion pumps
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F class pumps
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in mitochondria membrane, used in ATPase synthesis, thru oxidative phospho & krebs you pump H ions out of mito matrix increasing Hions out. This brings neg charges, creating energy storage (capacitor)
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Major info = reverse ATPases, use something going through to make ATP, also used in chloroplasts
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V class
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hydrolyzing ATP to drive ions through, pumping H into cells, relevant to lysosomes pumping H ions to lysosomes making them neg (but lysos use other prots to keep H from binding neg charges)
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ABC Superfamily
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consists of two transmembrane domains, two cytoplasmic ATP binding domains. Two examples include MDR1/CFTR1
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MDR1
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MultidrugResistantProtein - ATPase excretes hydrophobic compounds. Many toxins & drugs are hydrophobic, so detoxifies BUT also excretes drugs.
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CFTR1
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Differs from ABC family in two ways; 1 Functions as channel, not ATPase pump 2 Has regulatory domain, which has to be P'd by cAMP in addition to ATP hydrolysis. Significant in cystic fibrosis...
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E1&2 states apply to which groups?
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Transporters & P class ATPases
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E1 =
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Resting state conformation, high affinity site open
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E2 =
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closes high affinity site, opens low aff site - when factor is translocated to low site it is easily released
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Binding mechanism for P class ATPases?
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slightly differ by having separate ATPase domain for binding/hydrolyzing ATP. P from ATP to t-port protein forcing E1->E2 shift, factor translocates, dephosphs occurs and shift back to E1/release factor from low aff site
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Na/K ATPase used for?
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Electric potential & Na/K gradients; 3Na+ export: 2K+ imported for every ATP hydrolyzed
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Pseudomonas aeruginosa
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opportunistic bacteria associated with CF pts, lung infections etc
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