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11 Cards in this Set

  • Front
  • Back
In which direction is mRNA read?
5' to 3' (the same direction as it is synthesized)
In which direction are proteins synthesized?
From the amino terminus to the carboxyl terminus
(N to C)
What is the enzyme that "charges" or adds the amino acid onto tRNA?
Aminoacyl tRNA synthase.
Give a general layout of tRNA; how does it encode for a certain amino acid?
One side of the tRNA has a sequence that recognizes a codon and the other side of the tRNA carries the amino acid. This means that there is a specific tRNA for each amino acid.
What is a codon?

What is an anticodon?
A codon is the 3 nucleotide sequence that encodes for a particular amino acid (it is the code read by tRNA).

An anticodon is the complementary sequence of 3 nucleotides that match the codons on mRNA (it allows the tRNA to bind).
What is a wobble base?
The wobble base is the 3rd base in a codon.
What is the consequence of wobble bases?
It allows faster dissociation of tRNA.

It also (less important) contributes to overall specificity of tRNA.
Which 5'anticodon bases are allowed to pair to mRNA codons when each of the following are in the 3'codon position?
1) C
2) A
3) U
4) G
5) I
1) G only
2) U only
3) A or G
4) U or C
5) U, C or A
What makes up the majority of the mass in ribosomes?
The RNA (not the protein).
Where are eukaryotic ribosomes created?

Where are bacterial ribosomes created?
In the nucleolus.

Bacteria doesn't have a nucleus; the ribosomes are created in the cytoplasm.
What are the 3 tRNA binding sites on a ribosome?
A-site (aminoacyl-tRNA entry site)

P-site (peptidyl-tRNA site) [this is where amino acids are added to a peptide chain]

E-site (exit site)