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14 Cards in this Set
- Front
- Back
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What are the 5 steps of translation?
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Activation, Initiation, Elongation, Termination, and Post-Translation
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How is an amino acid activated?
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Bind with ATP
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Where and how does the amino acid bind to tRNA?
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Covalent (ester: very stable) bond - on acceptor stem
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What enzyme binds the aa to tRNA?
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aminoacyl-tRNA synthetase (ligase)
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What happens during activation?
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tRNA binds to an amino acid
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What is the first amino acid coded for on mRNA that binds to tRNA?
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f-Met (formyl Methionine)
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What does mRNA bind to in initiation?
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Small ribosome subunit (A site then P site)
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What hydrogen bonds to codon of mRNA in initiation?
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tRNA with anticodon for f-Met
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What does peptidyl-transferase do in elongation?
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Catalyzes formation of amide peptide bond
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In elongation, what is happening when the ribosome moves and tRNA goes from A-site to P-site?
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translocation
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What are the 3 stop codons in termination?
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UAA, UAG, UGA
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What is hydrolyzed in termination?
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ester bond from polypeptide to tRNA
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What is hydrolyzed post-translation?
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f-Met
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What forms during post-translation?
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RS---SR bonds (cysteine, disulfur)
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