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28 Cards in this Set
- Front
- Back
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Retrovirus advantages
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high efficiency
integration --> long term expression |
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Retrovirus disadvantages
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possible insert mutations
requires proliferating cells relatively small DNA insert |
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Lentivirus advantages
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No requirement for cell division
integration --> long term expression |
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Lentivirus disadvantages
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small insert
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Adenovirus advantages
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broad range of target cells
no requirement for cell division no integration --> no mutations |
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Adenovirus disadvantages
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transient residence in cells
immunogenic --> inflammation |
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Adenoassociated virus advantages
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both integration extrachromosomal maintainance
possible site specific integration |
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Adenoassociated virus disadvantages
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small transgene insert
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liposomes advantages
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no infection risk
no limit on DNA content potential for receptor targeting |
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liposomes disadvantages
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low efficiency
limitations on targeting transient expression lysomal uptake --> degradation |
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adv: high efficiency
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common retrovirus
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adv: integration --> long term expression
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retrovirus
lentivirus |
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adv: no requirement for cell division
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lentivirus
adenovirus |
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adv: broad range of target cells
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adenovirus
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adv: no integration --> no mutations
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adenovirus
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adv: both integration and extrachromosomal maintainance
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adeno-associated virus
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adv: possible site specific integration
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adeno-associated virus
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disadv: possibel insert mutations
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common retrovirus
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disadv: requires dividing cells
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common retrovirus
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disadv: relatively small DNA insert
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common retrovirus
lentivirus adeno-associated virus |
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adv: no infection risk
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synthetic vectors
liposomes nanoparticles |
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adv: no limit on DNA content
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synthetic vectors
liposomes nanoparticles |
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adv: potential for receptor targeting
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synthetic vectors
liposomes nanoparticles |
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disadv: low efficiency
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synthetic vectors
liposomes nanoparticles |
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disadv: limitations on targeting
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synthetic vectors
liposomes nanoparticles |
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disadv: lysomal uptake and degradation
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synthetic vectors
liposomes nanoparticles |
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disadv: transient expression
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synthetic vectors
liposomes nanoparticles Adenovirus (transient residence in cells |
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disadv: immunogenic inflammation
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adenovirus
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