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23 Cards in this Set
- Front
- Back
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Name the subunits of prokaryotic RNA Pol
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aplhax2
Beta Beta' Sigma |
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Which prokaryotic RNA subunit interacts with factors the bind upstream of the -35 box?
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alpha
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Which prokaryotic RNA subunit forms phosphodiester bonds?
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Beta
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Which prokaryotic RNA subunit binds the DNA template?
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Beta'
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Which prokaryotic RNA subunit recognizes the Pribnow box?
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Sigma
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Which prokaryotic RNA subunit facilitates initiation by opening the promoter complex?
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Sigma
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Where does Sigma factor bind?
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Only at promoters
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What prokaryotic RNA subunit replaces sigma factor?
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NusA
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What is the function of NusA?
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Assists in termination
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Describe Type I eukaryotic termination.
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Rho independent. Forms hairpin loop folowed by poly-A tail
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Describe Type II termination
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Involves Rho factor which breaks H bonds between DNA-RNA, and cleaves sequence before the poly-U stretch
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What is the function of the Rho factor?
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Termination. Catalyzes dissociation of RNA Pol
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How does Rifampicin work?
What are the side-effects? |
Increases hepatic enzyme activity of cytochrome P450. Hydroxylates compounds to assist in clearance by kidney.
Side-effects include hepatotoxicity and decreased effectiveness of drugs via dilution |
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How does Rifampicin inhibit bacterial transcription?
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Binds RNA Pol
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In eukaryotic transcription what is the function of RNA Pol I?
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Transcribes rRNA
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In eukaryotic transcription what is the function of RNA Pol II
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Transcribes mRNA
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In eukaryotic transcription what is the function of RNA Pol III?
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Transcribes tRNA and snRNA
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In eukaryotic transcription what is the function of the transcription factors (TFs)?
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Form a complex with RNA Pol II which effectively creates a holoenzyme
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Which TF inititiates complex formation and how does it do so?
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TFIID begins initiation by binding the TATA Box
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In eukaryotic transcription how is splicing terminated?
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By the formation of a lariat
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What is the structure of the lariat? How is it formed?
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2' to 5' phsophodiester bond formed at branch point sequence. lariat is removed and exons are fused
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What is the most common result of a splice site mutation concerning exons and why?
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Entire exon is skipped because they are freq recognized as units.
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List two diseases caused by splice site mutation
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Cystic fibrosis
Parkinson's Retinitis Pigmentosa Hemophilia B Myotonic dystrophy Cancer |
