Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
12 Cards in this Set
- Front
- Back
|
What is the effect of DNA methylation on transcription? How?
|
inhibition by stopping proteins from binding
|
|
|
There are 2 models for chromatin regulation during transcription initiation. 1. What is the first step?
2. What is the next steps of both models? |
1. HAT and chromatin remodelers to remodel chromatin
2. Model 1: histone eviction, initiation complex binds Model 2: Partial initiation complex, histone eviction, and Pol II and TFIIH join to complete the complex |
|
|
What are the two proteins involved in histone remodeling? Explain their function
|
FACT: composed of SSRP1 and Spt16.
-removes H2A/B dimer -destabilizes nucleosomes -allows RNAPII to traverse nucleosome SWR1: ATPase, exchange H2A for H2AZ(variant), which prevents spread of heterochromatin and gene silencing |
|
|
What are the features of chromatin when it is active/inactive wrt conformation, DNA methylation, histone acetylation
|
active chromatin: open, extended confomation, unmethylated promoter, acetylated histones
inactive chromatin, highly condensed, methylated promoter, deacetylated histones |
|
|
Which histones are known to be modified in transcription? How are they modified?
|
H2B Ub and H3 methylation
|
|
|
Acetylations on K9/14 on H3 recruit which protein for transcription?
|
TFIID
|
|
|
What are the TFs involved with RNAP II?
|
TFII B DEF HA
|
|
|
What is the difference between rho dependent and rho independent termination?
|
rho dependent- pol pauses at terminator, rho catches up. Rho unwinds RNA/DNA hybrid and terminates
rho independent- hairpin in RNA forms, pausing the RNAP, destabilizes RNA/DNA, falls off |
|
|
How is transcription terminated in RNAP I, II and III?
|
See last 3 slides of handout
|
|
|
What is U1 responsible for in splicing? How does it do it?
|
U1 defines 5' splice junction by base complementarity
|
|
|
What is U2 responsible for in splicing? How does it do it?
|
U2 defines the branchpoint A by base complementarity
|
|
|
What does Slu7 and U2AF do?
|
Select the 3' splice site (splice acceptor site)
|
