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101 Cards in this Set

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How does energy enter the ecosystem?

By light

What does photosynthesis generate?

Oxygen and organic molecules

How do cells generate ATP?

They use chemical energy stored in organic molecules

How do catabolic pathways yield energy?

By oxidizing organic fuels.

How do catabolic pathways work?

They release stored energy by breaking down complex molecules.

What does breaking down complex molecules do?

Release electrons

Is the breakdown of organic molecules is exergonic or endergonic?

Exergonic

What kind of energy is released in the breakdown of organic molecules?

Potential energy

What is fermentation?

A partial degradation of sugars that occurs without Oxygen

Wine, cheese, beer, bread

What are the 3 types of catabolic pathways to produce ATP?

Fermentation


Aerobic respiration


Anaerobic respiration

Aerobic and anaerobic respiration are both considered what?

Cellular respiration

Carbohydrates, fats, and proteins are all consumed as fuel.

What is involved in aerobic respiration?

Consumes organic molecules and Oxygen, yielding ATP.

What is anaerobic respiration?

Similar to aerobic respiration, but consumes compounds other than oxygen.

Organic compounds + oxygen —>

Carbon dioxide + water + energy

C6H12O6 + 6 O2 —> 6 CO2 + 6 H2O + energy (ATP and heat)

How is energy released in reactions?

The transfer of electrons releases energy stored in organic molecules.

What is released energy used for?

Synthesize ATP

What are redox reactions?

Chemical reactions that transfer electrons between reactants

What is LEO says GER?

Losing Electrons: Oxidation


Gaining Electrons: Reduction

What is Oxidation?

A substance loses electrons and is oxidized

What is reduction?

A substance gains electrons, or is reduced (the amount of positive charge is reduced)

In a redox reaction, what is the reducing agent?

The electron donor

What is the oxidizing agent in a redox reaction?

The electron receptor

Why do some redox reactions not transfer electrons?

They change the electron sharing in covalent bonds.

What is


Xe-

The electron donor


Become oxidized


Is the reducing agent (reduces Y)

What is


Y?

Accepts electron


Becomes Reduced


Is the oxidizing agent (oxidizes Xe-)

What are sources of high energy electrons?

Organic molecules with an abundance of H

How is energy released during CR?

As the electrons associated with H ions are transferred to O, a lower energy state

What happens during CR (redox)

Fuel (glucose) is oxidized


Oxygen is reduced

During the Stepwise Energy Harvest, electrons are transferred first from what, to where?

Electrons are transferred from organic compounds to NAD+, a coenzyme.

As an electron acceptor, what does NAD+ function as?

It functions as an oxidizing agent during CR

What does each NADH represent? And what IS NADH

Represents stored energy that is tapped to synthesize ATP


NADH is the reduced form of NAD

Where does NADH send electrons?

The ETC

How does the ETC pass electrons?

IN a series of steps

What function does Oxygen play in the ETC?

Plus electrons down the chain in an energy-yielding tumble

What are the 3 steps of CR?

Glycolysis


CAC


Oxidative Phosphorylation

What is glycolysis in CR?

Breaks down glucose into two molecules of pyruvate

What is the Citric Acid Cycle in CR?

Completes the breakdown of glucose

What is Oxidative Phosphorylation in terms of ATP production?

Powered by redox reactions of the electron transport chain


90% of ATP production

What is Substrate level Phosphorylation in terms of ATP production?

Enzyme transfers phosphate group from substrate to ADP


Less ATP produced via glycolysis and the CAC

How much ATP is produced for each glucose molecule?

Up to 32 ATP molecules

What is the purpose of Glycolysis?

Oxidizing glucose to pyruvate

What is Glycolysis?

Sugar Splitting

What does glycolysis do to one molecule of Glucose?

Splits one glucose into 2 pyruvate

Where does glycolysis occur?

In the cytosol

Is Oxygen needed for glycolysis?

Nope, doesn’t matter.

What types of cells support glycolysis?

Eukaryotic and Prokarytic

What are the 2 major phases o Glycolysis?

NRG investment phase


NRG payoff phase

What is the NRG investment phase?

Cell expends energy (2ATP)

What is NRG payoff phase?

Cell produces ATP is substrate level phosphorylation.

What is the Net Production from one glucose?

2 Pyruvate


2 ATP


2 H2O


2 NADH and 2 H+

What happens at the end of Glycolysis?

All of the C originally in glucose is found in the 2 molecules of pyruvate. NO C is released as CO2 in glycolysis

What happens to pyruvate in the presence of Oxygen?

It enters a mitochondrian

In Pyruvate Oxidation, what is completed?

Oxidation of glucose

What must happen before the CAC can begin?

Pyruvate needs to be converted to Acetyl Coenzyme A (Acetyl CoA)

What does pyruvate do in terms of Glycolysis and the CAC?

It links them

What is a function of Pyruvate Oxydation?

Converts Pyruvate to Acetyl CoA

How is Pyruvate oxidation carried out? (3)

1. Oxidation of pyruvate and release of CO2


2. Reduction of NAD+ to NADH


3. Combination of the remaining two carbon fragment and coenzyme A to form Acetyl CoA

Acetyl CoA can now enter the CAC after which cycle?

Pyruvate oxidation

What does the CAC do? Where does it occur?

Completes the breakdown of pyruvate to CO2 and it occurs in the Mito Matrix

What output does the CAC generate in one cycle?

1 ATP


3 NADH


1 FADH2

What happens to each pyruvate in the CAC?

It becomes 3 CO2


- one in Pyruvate oxidation


- two in CAC

How many steps does the CAC have?

8

How is citrate formed during the CAC?

The Acetyl group of Acetyl CoA joins the cycle by combing with oxaloacetate.

What do steps 2-8 in the CAC do?

Decompose the citrate back to oxaloacetate

What do steps 2-8 in the CAC do?

Decompose the citrate back to oxaloacetate

Where do the carbons in the CAC go after Acetyl CoA?

They become carbons in oxaloacetate in a subsequent cycle? And are lost as carbon dioxide in the subsequent cycle

What does each Acetyl CoA produce?

1 ATP


1 FADH2


3 NADH

What do NADH and FADH2 produced by the CAC do?

They relay electrons extracted from food to the ETC

What does the CAC produce per revolution?

1 ATP


3 NADH


1 FADH2

Most of the energy extracted from food comes from where?

NADH and FADH2

What does the ETC do?

Powers ATP synthesis via oxidative phosphorylation

Where does the ETC occur?

In the inner membrane of the mito

What is the first step of the ETC?

Electrons are transferred from NADH or FADH2 to the ETC

What are electrons passed through in the second step of the ETC?

Proteins including cytochromes. Each one with an iron atom to oxygen

What happens to the energy in electrons during the ETC?

Electrons drop in free energy as they go down the chain and are finally passed to O2, forming water

Are electron carriers reduced or oxidized states?

They alternate between reduced and oxidized states as they accept and donate electrons

What happens at the terminal electron acceptor?

Oxygen, which strongly electronegative then forms water

Does the ETC generate ATP?

Not directly

What happens to protons in the ETC?

They are pumped from the mito matrix to the inter membrane space

What does the ETC do in terms of protons?

Increases the concentration of protons in the inter membrane space.

What do the protons do once in the inter membrane space?

They move down the concentration gradient back across the membrane. And they pass through the protein complex ATP Synthase

What is Chemiosmosis?

The use of energy in a proton gradient to drive cellular work

HOw does Chemiosmosis work?

Proton moves into binding sites on the rotor of ATP synthase, causing it to spin and catalyze phosphorylation of ADP and inorganic phosphate > ATP

What are the first 2 of 5 steps of chemiosmosis?

1. Hydrogen ions flow down concentration gradient


2. Hydrogen ions enter building sites in rotor, changing shape of subunit so rotor spins within the membrane

What are steps 3 and 4 of 5 in chemiosmosis?

3. Each proton makes one spin before exiting via a channel into the mito matrix


4. Spinning of rotor causes internal rod to spin

What is the 5th step in chemiosmosis?

Spinning rod causes catalytic sites in the catalytic knob to produce ATP from ADP and P

What is the sequence of energy flow during CR?

Glucose -> NADH -> ETC -> proton motive force -> ATP

How much of the energy in a glucose molecule is transferred to atp?

34% making 32 ATP

What is oxidative phosphorylation and what does it contain?

ETC and Chemiosmosis

After what cycle and in the presence of Oxygen do cells use aerobic respiration?

Glycolysis

What makes electrons move down the ETC?

Electronegative Oxygen

Does anaerobic respiration use an ETC?

Yes, but it does not use oxygen as final electron acceptor, so it doesn’t produce water at the end

What is fermentation?

Uses substrate level phosphorylation instead of an ETC to generate ATP


Consists of glycolysis plus reactions that regenerate NAD+ which can be reused by glycolysis

What are the 2 types of fermentation?

Alcohol fermentation


Lactic acid fermentation

What happens in alcohol fermentation?

Pyruvate is converted to ethanol in 2 steps

What are the 2 steps of AF

Release CO2 from Pyruvate


Produce NAD+ and ethanol

What happens in LAF?

Pyruvate is reduced by NADH forming NAD+ and lactate as end products with no release of CO2

What do Fermentation, Aerobic Respiration, and Anaerobic Respiration have in common?

Glycolysis. Used to oxidize glucose and harvest the chemical energy of food.

In fermentation, what acts as the final electron acceptor?

Organic molecule, pyruvate or acetaldehyde

How many ATP does Fermentation produce?

2

How many ATP are produced in CR?

32 ATP