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72 Cards in this Set
- Front
- Back
- 3rd side (hint)
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The catalase test does what?
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differentiates b/w these two bacteria: staphylococci and streptococci
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"cata" is to "lase" as ______ and ______ are to "ococci"
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Is always catalase +
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staphylococci
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positively loco
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Is always catalase -
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streptococci
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has a reputation for being negative
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The catalase test uses what?
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hydrogen peroxide (h202)
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A positive catalase tests yields:
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bubbles!
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This agar contains catalase
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blood agar - RBCs contain catalase
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These tests are designed to test an organism against a known substance
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Sensitivity tests
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If it is susceptible to Bacitracin, it must be
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S. pyrogenes (GAS)
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Bacitracin whips GAS ASS!
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If it is resistant to Bacitracin, it must be
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Unknown beta-hemolytic Strep
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Strep's not done yet!
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If it is resistant to Optochin, it must be
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S. viridans
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What rhymes with chins?
S. viridans whips optochin |
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If it is susceptible to Optochin, it must be
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S. pneumoniae
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If it is susceptible to Novobiocin, it must be
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S. epidermidis
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If it is resistant to Novobiocin, it must be
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S. saprophyticus
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This test differentiates GBS from other beta-hemolytic streptococci:
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CAMP test
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A positive CAMP test is indicated by
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an arrowhead-shaped area of enhanced hemolysis
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A positive CAMP test tells you that the suspect is
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S. agalactiae
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This test helps to differentiate Group D Streptococci and E. faecalis from other streptococci
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Bile Esculin test
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Group Death Strep and E. Fatality are found with the Black Evil Test
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Group D Streptococci is also known as
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S. bovis
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Death cattle
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is possibly involved in colon cancer
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S. bovis / Group D Strep
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Death Strep
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A positive Esculin test results in
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blackening of the agar medium
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Black Evil test: black agar
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A positive Esculin test reveals that what must be present
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either Group D Strep (S. bovis) or E. faecalis
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Positive Evil test: Group death or fatalist
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This test separates S. aureus from all other Staph species
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Coagulase test
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coagulase -> a from agglutination -> staph aureus
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produces a coagulase enzyme that is involved in clotting
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S. aureus
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A positive Coagulase test yields
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clumping, like a snowflake
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A negative Coagulase tests yields
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no clumping
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The Coagulase test mixes the bacteria with
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rabbit plasma
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Coagulase initiates the terminal clotting sequence and converts ________ to ____________.
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fibrinogen to fibrin
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What kind of medium is MSA?
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selective/differential
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The Mannitol Salt Agar test separates other bacteria from
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S. aureus
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A positive MSA test yields
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yellow agar
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This test detects c-component of the cytochrome-oxidase complex
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Oxidase test
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The Oxidase test is important in identifying which bacteria?
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Neisseria, Pasteurella, Pseudomonas
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A positive Oxidase test results in a color change from clear to
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purple
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This test differentiates H. influenzae from other Haemophilus species
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X- and V-factor test
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aka Heme
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X-factor
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aka NAD
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V-factor
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H. influenzae will only grow in the presence of
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both X- and V-factors
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a cheap X- and V-factor like test
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Satellite test
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The bacteria used in the Satellite test
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S. aureus
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The 5 members of the Enterobacteriaceae family:
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E. coli, Shigella, Salmonella, Klebsiella, Proteus
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TSI Agar test is used to differentiate b/w those that ferment and those that do not ferment what?
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glucose
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TSI only differentiates b/w which type of bacteria?
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Gram-negative rods
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the TSI test also differentiates b/w producers and non-producers of
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H2S
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This test is important in identifying the Enterobacteriaceae family
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TSI Agar test
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TSI is like TMI -> diarrhea!
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red pH indicator color means
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Alkaline
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yellow pH indicator color means
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acidic
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like pee
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If the result of a TSI test is red butt and red slant, then
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the bacteria cannot utilize the carbohydrates
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If the result of a TSI test is yellow butt and red slant, then the organism can
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ferment glucose to produce acids (pH lowers), then the bacterium breaks down peptone to produce ammonia (pH raises)
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TSI test result: yellow butt and yellow slant means
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ferments glucose, sucrose, and or lactose
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TSI test result of blackening in the butt is indicative of what being produced from thiosulfate?
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H2S
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In TSL agar, H2S reacts with iron salt and precipitates in the form of
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ferrous sulfide (black)
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Tests the ability of the organism to produce indole from tryptophane
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Indole test
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ALWAYS indole positive
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E. coli
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A positive Indole test yields a
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red-colored ring
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A negative Indole test yields a
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yellow-colored ring
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the chemical used in the indole test
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Kovacs reagent
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a negative Urease test is what color?
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yellow
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a positive Urease test is what color?
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red (from light pink ro rosy to bright magenta), depending on the strength of the urease
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A weak urease producer
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Klebsiella
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A strong urease producer
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Proteus
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causes ulcers
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H. pylori
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An ultra-strength urease producer
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H. pylori
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A useful test of differentiation among glucose-nonfermenters
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Nitrate reduction test
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All members of this family reduce NO3 to NO2
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Enterobacteriaceae family
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UTI's are diagnosed by
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elevated urine nitrite
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If NO2 was produced, it yields what color compound in the Nitrate reduction test?
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red
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reduces NO3 to N2
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Pseudomonas aeruginosa
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All members of the Enterobacteriaceae family are motile, except
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shigella & klebsiella
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most of these are non-motile
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Gram-positive organisms
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some representatives of ________ genus are motile
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Bacillus
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L. monocytogenes is motile, but only at
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room temperature
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how do we distinguish L. monocytogenes from GBS?
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it is motile at room temperature
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