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22 Cards in this Set

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Histology
Study of cells and the extracellular matrix
Resolution
a measure of the amount of detail in an image
Magnification
process of enlarging something only in appearance, not in physical size.
Fixation
Pieces of organs are treated to avoid tissue digestion via autolysis or bacteria to preserve molecular composition.
Fixative
Solutions of stabilizing or cross-linking agents used in chemical fixation.
Microtome
Instrument used for sectioning paraffin-embedded tissue for light microscopy.
Acidophilic
Cationic components (proteins with many ionized amino groups) have affinity for acidic dyes.
Basophilic
Anionic components stain more readily with basic dyes.
Hematoxylin
Stains DNA of cell nucleus and other acidic structures (RNA-rich portions of cytoplasm and matrix of catrilage) blue.
Eosin
In contrast to Hematoxylin, stains other cytoplasmic components and collagen pink.
H&E
Hematoxylin and eosin; most common combination of dyes.
Trichrome
Show neuclei and cytoplasm. Help to distinguish extracellular tissue components better than H&E. e.g. Mallory stain, Masson stain.
Periodic acid-Schiff (PAS)
Used in Fuelgen reaction to identify and quantify DNA by hydrolysis of deoxyribose.
Freeze fracture
Prep method useful for examining lipid membranes and their incorporated proteins in "face on" view.
Freeze etch
Replica of specimen is made from sample that has been rapidly frozen and then fractured along natural planes of weakness to reveal its internal structure. Prep for electron microscope.
Histochemistry
branch of science that deals with the chemical composition of the cells and tissues of the body.
Immunocytochemistry
common laboratory technique that uses antibodies that target specific peptides or protein antigens in the cell via specific epitopes.
Autoradiography
technique using X- ray film to visualize molecules or fragments of molecules that have been radioactively labeled.
Fluorescence
When certain substances are irradiated by light of a certain wavelength, they emit light with a longer wavelength.
Electron Microscopy
microscopy technique that uses a beam of electrons to illuminate the specimen and produce a magnified image.
Transmission electron microscopy (TEM)
microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through.
Scanning electron Microscopy
type of electron microscopy that images a sample by scanning it with a high-energy beam of electrons in a raster scan pattern.