Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
19 Cards in this Set
- Front
- Back
|
Restriction enzymes
|
are bacterial proteins that cleave DNA at specific sequences.
|
|
|
GAATTC
|
is a palindrome and a restriction site.
|
|
|
DNA is negatively charged.
|
True
|
|
|
DNA is separated on gel electrophoresis based on:
|
molecular weight.
|
|
|
Southern Blot
|
is used to identify DNA.
|
|
|
Northern Blot
|
is used to identify RNA.
|
|
|
Western Blot
|
is used to identify protein.
|
|
|
A probe
|
is defined as a single-stranded piece of DNA or RNA that is used to visualize complementary sequences within DNA.
|
|
|
DNA sequencing
|
utilizes dideoxy nucleotides to sequence segments of DNA.
|
|
|
PCR
|
stands for polymerase chain reaction and is a technique used to amplify DNA.
|
|
|
Taq polymerase
|
is derived from the bacteria Thermus Aquaticus commonly found in hot springs.
|
|
|
A gene knockout
|
is a technique used by researchers to knockout a particular gene of interest.
|
|
|
A karyotype
|
is a technique used to identify DNA chromosomal abnormalities. For example, trisomies, monosomies, and various other abnormalities can be detected by karyotypes.
|
|
|
Cloning
|
is a technique used to create an exact copy of oneself.
|
|
|
RNAi
|
inhibitor RNAs
|
|
|
RNAi
|
is responsible for degrading specific mRNAs
|
|
|
Dicer
|
is the enzyme responsible for cleavage of RNAi into siRNA.
|
|
|
RISC
|
stands for RNA induced silencing complex and is the enzyme responsible for cleaving the endogenous mRNA.
|
|
|
List the diseases currently being investigated for RNAi treatment.
|
Macular Degeneration, Hypercholesterolemia, RSV, Hepatitis C, and various others are potential diseases being investigated as potential RNAi targets.
|
