Tca Cycle

Front 1

Back 1

Front 2

Back 2

Front 3

Back 3

Front 4

Back 4

Front 5

Back 5

Front 6

Back 6

Front 7

Citrate synthase transfers the ___________ group from acetyl-CoA to __________.

Back 7

Citrate synthase transfers the acetylgroup from acetyl-CoA to oxaloacetate.

Front 8

The citrate synthase mechanism usesacid/base catalysis to form an enol intermediate followed by hydrolysis of the ____________ bond.

Back 8

The citrate synthase mechanism usesacid/base catalysis to form an enol intermediate followed by hydrolysis of thethioester bond.

Front 9

The citrate synthase mechanism usesacid/base catalysis to form an _________ intermediate followed by hydrolysis of thethioester bond.

Back 9

The citrate synthase mechanism usesacid/base catalysis to form an enol intermediate followed by hydrolysis of thethioester bond.

Front 10

The citrate synthase mechanism uses ______________ to form an enol intermediate followed by hydrolysis of thethioester bond.

Back 10

The citrate synthase mechanism usesacid/base catalysis to form an enol intermediate followed by hydrolysis of thethioester bond.

Front 11

_________________ interconverts citrate and isocitrate.

Back 11

Aconitase interconverts citrate and isocitrate.

Front 12

Aconitase interconverts _________ and ____________.

Back 12

Aconitase interconverts citrate and isocitrate.

Front 13

Back 13

Front 14

Back 14

Front 15

Back 15

Front 16

Back 16

Front 17

Isocitrate DH catalyzes the ____________ _____________ of citrate, forming α-ketoglutarate using Mn2+ as a cofactor.

Back 17

Isocitrate DH catalyzes the oxidative decarboxylation of citrate, forming α-ketoglutarate using Mn2+ as a cofactor.

Front 18

Isocitrate DH catalyzes the oxidative decarboxylation of _____________, forming ______________ using Mn2+ as a cofactor.

Back 18

Isocitrate DH catalyzes the oxidative decarboxylation of citrate, forming α-ketoglutarate using Mn2+ as a cofactor.

Front 19

Isocitrate DH catalyzes the oxidative decarboxylation of citrate, forming α-ketoglutarate using _____________ as a cofactor.

Back 19

Isocitrate DH catalyzes the oxidative decarboxylation of citrate, forming α-ketoglutarate using Mn2+ as a cofactor.

Front 20

Back 20

Front 21

Back 21

Front 22

Back 22

Front 23

Back 23

Front 24

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Back 24

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Front 25

The __________________________ converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Back 25

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Front 26

The α-ketoglutarate DHcomplex converst ____________ to _____________, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Back 26

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Front 27

The α-ketoglutarate DH complex converst α-ketoglutarate to succinyl-CoA, using cofactors ____, _________, _______, _________ and __________.

Back 27

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

Front 28

The α-ketoglutarateDH complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+. Which enzymes are identical in both the pyruvate DH and the α-ketoglutarate DH?

Back 28

The α-ketoglutarate HD complex converst α-ketoglutarate to succinyl-CoA, using cofactors TPP, lipoamide, CoA, Fad and NAD+.

E3 is the only identical one as it restores the lipoamide from the dihydrolipoamide.

Front 29

Succinyl-CoA synthetase couples hydrolysis of succinyl-CoA's thioester bond to phosphorylation of _________ to make succinate.

Back 29

Succinyl-CoA synthetase couples hydrolysis of succiyl-CoA's thioester bond to phosphorylation of GDP to make succinate.

Front 30

Succinyl-CoA synthetase couples hydrolysis of ____________ ___________ bond to phosphorylation of GDP to make succinate.

Back 30

Succinyl-CoA synthetase couples hydrolysis of succiyl-CoA's thioester bond to phosphorylation of GDP to make succinate.

Front 31

________________________ couples hydrolysis of succinyl-CoA's thioester bond to phosphorylation of GDP to make succinate.

Back 31

Succinyl-CoA synthetase couples hydrolysis of succiyl-CoA's thioester bond to phosphorylation of GDP to make succinate.

Front 32

Succinyl-CoA synthetase couples hydrolysis of succiyl-CoA's thioester bond to phosphorylation of GDP to make _______________.

Back 32

Succinyl-CoA synthetase couples hydrolysis of succiyl-CoA's thioester bond to phosphorylation of GDP to make succinate.

Front 33

Back 33

Front 34

Back 34

Front 35

Back 35

Front 36

Back 36

Front 37

Back 37

Front 38

Which enzyme uses FAD as the cofactor?

Back 38

Succinate DH

Front 39

Which enzyme is bound in the membrane of the mitochondria and is a part of ETC complex 2?

Back 39

Succinate DH

Front 40

Succinate dehydrogenase catalyzesoxidation of ____________ to produce ____________ (and FADH2).

Back 40

Succinate dehydrogenase catalyzes oxidation of succinate to produce fumarate (and FADH2).

Front 41

_____________________ catalyzes oxidation of succinate to produce fumarate (and FADH2).

Back 41

Succinate dehydrogenase catalyzes oxidation of succinate to produce fumarate (and FADH2).

Front 42

Succinate dehydrogenase catalyzes oxidation of succinate to produce fumarate (and ________).

Back 42

Succinate dehydrogenase catalyzes oxidation of succinate to produce fumarate (and FADH2).

Front 43

Back 43

Front 44

Back 44

Front 45

Back 45

Front 46

_______________ catalyzes the hydration of fumarate's C=C bond to produce malate.

Back 46

Fumarase catalyzes the hydration of fumarate's C=C bond to produce malate.

Front 47

Fumarase catalyzes the hydration of fumarate's _____ bond to produce malate.

Back 47

Fumarase catalyzes the hydration of fumarate's C=C bond to produce malate.

Front 48

Fumarase catalyzes the hydration of _________ C=C bond to produce __________.

Back 48

Fumarase catalyzes the hydration of fumarate's C=C bond to produce malate.

Front 49

Back 49

Front 50

Back 50

Front 51

Back 51

Front 52

Malate DH oxidizes __________ to regenerate ____________ and produce NADH.

Back 52

Malate DH oxidizes malate to regenerate oxaloacetate and produce NADH.

Front 53

Malate DH ____________ malate to regenerate oxaloacetate and produce NADH.

Back 53

Malate DH oxidizes malate to regenerate oxaloacetate and produce NADH.

Front 54

_______________ oxidizes malate to regenerate oxaloacetate and produce NADH.

Back 54

Malate DH oxidizes malate to regenerate oxaloacetate and produce NADH.

Front 55

Malate DH oxidizes malate to regenerate oxaloacetate and _____________ _________ .

Back 55

Malate DH oxidizes malate to regenerate oxaloacetate and produce NADH .

Front 56

Back 56

Front 57

Back 57

Front 58

Back 58

Front 59

Back 59

Front 60

Back 60

D

Front 61

Back 61

D

Front 62

Which steps are irreversible in TCA cycle?

Back 62

1,3,4

Aconitase

Isocitrate DH

α-ketoglutarate DH

Front 63

Which enzmes produces reduced electron carriers in TCA cycle?

Back 63

Isocitrate DH

α-ketoglutarate DH

succinate DH

Malate DH

Front 64

Which enzmes produces reduced electron carriers in TCA cycle?

Back 64

Isocitrate DH

α-ketoglutarate DH

succinate DH

Malate DH

Front 65

Which enzymes release CO2 as a byproduct?

Back 65

Isocitrate DH

α-ketoglutarate DH

Front 66

Back 66

A

Front 67

Back 67

A