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37 Cards in this Set

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What is Asepsis?
Prevention of microbial contamination of living tissues or sterile materials by excluding, removing or killing micro-organisms including pathogens and spores.
What is Aseptic technique?
Refers to a procedure that is performed under sterile conditions to prevent nosocomial infections. It breaks the cycle of infection which limits the patient's exposure to a number of microorganisms that is not detrimental.
What is the Cycle of Infection?
Pathogenic microorganism --> reservoir or source --> portal of exit --> method of transmission --> portal of entry (surgical wound) --> susceptible host --> (keeps circling)
Why do we need surgical asepsis?
The act of surgery robs a patient of a protective barrier (skin) against infection or disease. Whenever the skin is disrupted, microorganisms have the opportunity to invade tissue and proliferate. Therefore, it is a must to practice surgical asepsis during a surgery.
What is a pathogen?
Microorganisms that cause infection or disease in humans and animals.
What is an infection?
It is the multiplication of an infectious agent within the body tissues that results in cellular injury.
What is a nosocomial infection?
An infection that is the result of treatment in a hospital. Considered nosocomial if appears in 48 hours or more in the hospital or within 30 days of discharge.
Source of Organisms?
Exogenous: operating room environment (air), surgical instruments, and surgical team.

Endogenous: Patient's resident flora = MOST IMPORTANT SOURCE
What is the most common form of nosocomial infection?
Surgical Site Infection (SSI)
Describe surgical site infections.
Purulent discharge

Signs of inflammation (redness, swelling, heat, pain, loss of function)

Isolation of microorganisms from wound.
What are the clinical significances of SSI?
Can lead to increased morbidity and possibly mortality.

Can progress to Systemic Inflammatory Response Syndrome (SIRS)

Very costly to treat.
Surgical asepsis involves proper preparation of what?
1. Facilities and environment
2. Surgical site
3. Surgical team
4. Surgical equiment
Why must surgical personnel wear proper attire?
Provides a barrier between sources of contamination - the patient and personnel
Where must hands be for surgical asepsis and why?
Hands are clasped in front of body above waist (may not be folded into axillary region)

The axillary region of the gown is not considered sterile.
Where are gowns considered sterile?
Mid chest to waist and from gloved hand to 2 inches above elbow.

Object held below a person's waist is considered contaminated.

Can't see what is touched. The back of the gown is not considered sterile.
Why must surgical team members remain within the sterile area?
To minimize contamination.

Cross contamination: The transfer of an infection directly from one person to another or indirectly from one person to a second person.
Why can't you talk, sneeze, laugh or cough over a sterile field or objects?
To minimize the moist droplets laden with bacteria from these sources.
Why are sterile tables only sterile at table height?
Items hanging over the table edges are considered non-sterile because they are out of the surgeon's vision.
What is used to create a sterile field?
Sterile drapes. Drapes should be waterproof. Should not be moved or rearranged once positioned.
Why must equipment used during surgery be sterilized?
To prevent a source of contamination.
Why must all items within a sterile field be sterile?
Contamination can occur from perforated wrappers or from strike-through from moisture transport.
How should items be introduced onto the sterile field?
Open, dispensed, and transferred by methods that maintain sterility. The edges of sterile containers are not considered sterile once package is opened and once a fluid cap has been removed, it cannot be replaced on the bottle.
If a sterile object touches the sealing edges of the pouch that holds it during opening, it is considered contaminated. Why?
Once opened, sealed edges of the pouches are not sterile.
Why should non-scrubbed personnel not reach over the sterile fields?
To prevent the contamination from dust, lint, exfoliated skin or other vehicles of bacteria that may fall on the sterile field.
Why should a sterile field be maintained and monitored constantly?
Diligent observation is an important part of infection control and must be exercised by all members of surgical team.
How should scrubbed team members stand and why?
Should always face each other and the sterile field at all times. Never turn your back on a sterile tray or leave it unattended. This prevents contamination from the backside of the team member which is normally considered non sterile.
How much movement should occur for surgical asepsis?
Movement in the operating room by all personnel is kept to a minimum except when needed to prevent cross contamination from turbulent air flow.
How should personnel moving within or around a sterile field do so?
When scrubbed personnel change positions, they should maintain a safe distance and pass each other back-to-back or front-to-front.
Who should handle sterile items and why?
Scrubbed personal handle only sterile items. (Non-scrubbed personnel handle only non-sterile items). This prevents cross contamination.
If the sterility of an item is questioned, what should you do?
Consider it contaminated. A sterile object becomes contaminated by prolonged exposure to air. This is to prevent cross contamination.
A sterile object or field becomes contaminated by capillary action when a sterile surface comes in contact with a wet contaminated surface. Why?
Moisture carries bacteria from a non-sterile surface to a sterile surface (strike-through).
Why should surgical team remain seated until the surgery has been completed if they began the surgery seated?
The surgical field is sterile only from table height to the chest; movement from sitting to standing during surgery may increase cross contamination.
What are the main portals of exit from human reservoirs for the cycle of infection?
Hands, mouth, nose and secretions.
What are the methods of transmission for cycle of infection?
1. Direct contact by hands to animal, animal to animal, object or person or animal
2. Airborne
3. Water droplets
4. Vectors
What can the portals of entry be for cycle of infection?
1. Mouth - GI tract
2. Nostrils - Respiratory tract
3. Break in skin
What 3 main factors determine whether a pathogen is successful in establishing an infection?
1. Susceptibility of host
2. Amount of pathogen
3. Presence of all elements of the infection cycle
Aseptic technique is a process designed to break the infection cycle. It includes....?
1. Practices that reduce the number of organisms by interrupting the cycle at the level of causative agent and reservoir.
a. Use of antiseptic soap (hand washing and patient skin preparation.
b. Disinfectants on objects
c. Sterilization of instruments/drapes

2. Practices that interrupt transmission at the level of portal of exit and entry
a. Gloves, gowns, mask, surgical drapes