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24 Cards in this Set
- Front
- Back
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Actin: G and F forms
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G actin is the monomer form of the polymer F actin. The addition and removal of G actin subunits to an actin chain is known as treadmilling.
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Cytochalasin D
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Cytochalasin D is an alkaloid drug that prevents polymerization of actin by binding to the monomeric form. Depolymerization then occurs as the concentration of free monomeric actin decreases.
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Formalin
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Formalin is a solution of formaldehyde. It functions to fix cells, by halting all cellular processes.
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DAPI
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DAPI is a stain that targets the cell nucleus because of its affinity to the AT regions of DNA.
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FITC
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FITC is a fluorescent stain that was used in conjunction with phalloidin in this experiment to illuminate the polymerized F actin.
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Phalloidin
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Phalloidin is a toxin extracted from death cap mushroom that aggressively binds to F actin preventing depolymerization.
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Saponin
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Saponin is a detergent used to permeabilize the cellular membrane.
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Plasmid
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Plasmids are DNA molecules that can introduce genetic information to the host cell.
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Bluescript/KS
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pBluescript is a plasmid commonly used in cloning because of its multiclonal region and the presence of a lacZ region and ampicillin resistance.
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Supercoiled; Nicked; Linear DNA
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These are three forms of plasmid DNA that have different running speeds on an electrophoresis gel: Nicked DNA has one strand cut running slowest, Linear DNA has free ends and has a median running speed, Supercoiled DNA is fully intact with both strands uncut, and with a twist built in making it compact and run quickest.
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Phenol/Chloroform
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Phenol/Chloroform created a layered solution with proteins below and plasmid DNA above.
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Agarose Gel
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Agarose gel is commonly used for electrophoresis. Agarose dissolved in PBS creates a sieve like material trapping larger pieces of DNA as an electrical charge attracts them to the opposite end of the gel.
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RNase
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Rnase is an enzyme that degrades RNA into individual base pairs.
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DNase
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Dnase is an enzyme that degrades DNA into individual base pairs.
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Restriction endonucleases/enzyme
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Restrictions cut DNA strands at specific locations creating strands with complementary ends.
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Recombinant vector
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A vector is a plasmid that has been engineered to readily accept foreign DNA via recombination.
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HELA cells
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HeLa cells are a human tumor cell line originally derived from a cervical carcinoma over 40 years
ago. |
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Spectrophotometry
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The use of absorbed light to determine the concentration of a substance in solution.
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Fluorometry
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The use of emitted light to determine the concentration of a substance in solution. Light is shined on the sample at a particular wavelength and the reflected light is measured.
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Bradford assay
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The use of the linear relationship between protein concentration and the increase in the absorbance of
an added dye solution at 595 mn. |
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BSA
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Bovine Serum Albumin (BSA) is a standard of measurement that is used to create a standard curve.
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How was the concentration of proteins determined in Lab 3?
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A Bradford assay.
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How was the concentration of total nucleic acids determined in Lab 3?
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Spectrophotometry.
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How were the individual concentrations of RNA and DNA determined in Lab 3?
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Fluorometry was used to determine the concentration of DNA. Then the concentration of DNA was subtracted from that of total nucleic acids.
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