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30 Cards in this Set

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Sterilization
The complete destruction of pathogens including endospores
Sterilization
The complete destruction of vegetative forms of microoganisms
antisepsis
the application of chemical agents to a surface to inhibit microbial growth and reproduction
steam autoclaving
combines heat, pressure, and moisture
Steps in equipment processing
1) Equipment is disassembled
2)washed in detergent
3) rinsed
4) Loosely packaged
5) steamed
Critical factors in steam autoclaving
exposure time, pressure, and temperature
Temp and pressure for steam autoclaving
121 degrees c for 15 minutes at 15 psi, or 121 c for 3 minutes at 30 psi
Disadvantages of steam autoclaving
1) dulls surgical instruments
2) Speeds deterioration of rubber and some plastics
3) contraindicated for some electronics
Advantages of pasteurization
1) does not destroy plastics
2) Uses only water
3) destroys vegetative organisms
4) 77 degrees c for 30 minutes
Etheylene Oxide Processing
1) Toxic gas combined with moisture and heat
2) effectiveness depends on equipment preparation, gas concentration, humidity, and temperature
Steps in Ethylene Oxide processing
1) Diassemble
2)wash in detergent
3) rinse
4) dry
4) package in poroous packages
Ethylene oxide concentration
Shouuld be between 800 and 100 mg/L
Upper critical temp for ethylene oxide processing
no higher than 60 (should be between 49 and 57)
Humidity range for ethylene oxide processing
between 49 and 57 degrees C
Contraindications for ethylene oxide processing
Not for plastics
necessary aeration time
Glutaraldehyde processing
chemical that is used to cold sterilize or disinfect equipment by immersion
How are organisms destroyed in gluteraldehyde processing
By alkylation of forms
Time limits for gluteraldehyde processing
1) 10-20 minutes for disinfection
2)6-10 hours for sterilization
Steps in gluteraldehyde processing
1) Immersion
2)rinse with sodium bisulfite
3) allow to dry in filter cabinet
Contraindications of gamma irradiation
1) expensive
2) cumbersome
Bacteriological Surveillance
Provides data on the effectiveness of disinfection and sterilization methods
Output sampling
Aerosol directed through a funnel to a culture plate
Rinse Sampling
sterile broth is exposed to respiratory therpay equipment, then cultured
Rodec Plate Sampling
plate is pressed against equipment, then cultured
Swab Sampling
Swab is exposed to RT equipment, then plated, then cultured
Common Methods of Heat sterilization
1) incineration
2) Dry heat
3) boiling
4) autoclaving
Dry heat sterilization technique
1) objects placed in oven for 1-2 hours at temps between 120 and 180 degrees C
2) ideal for glass and metal
Boiling
1) kills all vegetative bacteria and most viruses in 30 minutes
Autoclaving
1) efficient
2) quick
3) cheap
4) clean
Autoclaving temperatures, time, and pressure
20 psi, 126 degrees C, 10 minutes