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18 Cards in this Set
- Front
- Back
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what to do when materials can't stand up to autoclave
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tyndallize - flow hot steam over materials, slower, fractional
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3 ways to use hto steam for sterilization:
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-autoclave
-tyndallize -inspissation |
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inspissation
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thickening through evaporation; used on mycobacteria's high-protein media that can't be autoclaved
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most common type of filter for sterilizing
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millipore
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what is filtering used for?
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sterilizing things that can't be heated like body fluids, urea, carbs.
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most frequent chemical used for sterilizing: what materials?
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ethylene oxide for plastic petri dishes, pipettes, etc.
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definition of disinfectant
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killing of vegetative organisms; not necess. spores!
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what interferes with disinfectants?
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PROTEIN; must denature w/ detergent before using disinfectant.
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what test evaluates the activity of other disinfectants?
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the PHENOL COEFFICIENT TEST; its such a powerful disinfectant that it holds the bar high
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definition of ANTISEPSIS
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killing/inhibiting of organisms on the skin. doesn't hurt the tissue, so milder than disinfectant.
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2 common types of Antiseptics
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-mercurochrome
-tincture of iodine |
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2 classes of chemicals:
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A. affect surface layers of cell
B. denature cellular proteins, thus reduce enzyme activity |
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what do surface active chemicals do?
examples? |
affect cell membrane permeability.
quaternary ammonium compounds |
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3 chemicals that denature cell membrane
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-phenols
-cresols -bis-phenols LYSOL is a cresol |
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4 chemicals that denature cellular proteins and therefore reduce enzyme activity
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-acid
-alkali -alcohol -acetone |
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last five chemicals that denature cell proteins:
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-heavy metals -mercury, arsenic, silver
-oxidizing agents (iodine/chlorine, h2o2, permanganate) -formaldehyde -ethylene oxide -dyes - crystal violet! |
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action of lysol
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denatures the cell membrane
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does tyndallization kill spores?
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no; so you do it for 30 min on 3 consecutive days to allow spores to germinate between tyndalilizations
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