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15 Cards in this Set
- Front
- Back
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Staphylococci |
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Staphylococci gram test |
Gram positive |
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Staph arrangement |
Bunches,non motile,non-capsulated,non spore forming |
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Classification according to |
Coagulase enzyme production Endopigment production |
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Coagulase positive example |
Staph. Aureus |
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Coagulase negative example |
Staph. Epidermis Staph. Saprophyticus |
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Endopigments |
Staph. aureus: golden yellow Staph. albus (epidermidis): white Staph. citrus: lemon yellow |
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Classification of diseases caused by Staph. Acc to |
Caused by organism itself Caused by toxins produced |
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Invasive infections-> due to..? May be...? |
Due to organism itself May be 1)superficial infections (skin infections) 2)deeply seated (inside body-> bacteremia, UTI, diarrhoea) |
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Diseases due to toxins |
1)Scalded skin syndrome (SSS) 2)Toxic shock syndrome (TSS) 3)Staphylococci food poisoning |
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Blood agar plate isolation |
Staph. aureus -> ß haemolysis Coagulase negative staphylococci-> no hemolysis |
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Selective media |
Mannitol salt agar: a selective differential media used to differentiate between Stapylococcus aureus and coagulase negative Staph. |
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Composition |
7.5% NaCl: makes the medium selective for Stapylococcus since most bacteria cannot tolerate this high level of salinity. Mannitol: the sugar to be tested Phenol red: pH indicator |
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Mannitol salt agar result |
The development of yellow zones around the colonies is an indication that mannitol has been fermented with the production of acid which changes the colour of the indicator (yellow for aureus only) |
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Biochemical tests |
Catalase test Coagulase test |
