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27 Cards in this Set
- Front
- Back
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family/genus/species of staph:
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micrococcaceae
staphylococcus aureus, epidermidis, saprophyticus, +13 |
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gram stain of staph
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nice round gram positive cocci in clusters
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catalase test for staph shows
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positive
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growth requirement of Staph
type of respiration |
none/ not fastidious
facultative anaerobe - grows anywhere |
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colonial morphology of all staph species
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white, round, creamy, opaque, convex, large with prolonged incubation
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coag test for staph
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positive; if slide shows positive you're good.
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principle of coag test
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first do slide test for bound coag; if negative, then do a tube test for free
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pigmentation of staph aureus vs. epi
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aureus = yellow, epi = white
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hemolytic rxtns of aureus vs. epi
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aureus = beta
epider = gamma |
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principle of latex agglutination test
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latex beads coated with
-latex -anti-protein A bugs that produce coagulase will bind beads. bugs that have protein A (s aureus does) will bind Ab. |
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alternative tests for I.D. of aureus or epi
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latex agglutination
DNAse or Thermostable Nuclease Mannitol salt Novobiocin |
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principle of novibiocin test
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saprophyticus is resistant, other species of staph are not
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what type of specimen is mannitol salt for
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stools
shows fermentors - s. aureus shows growth if able to grow on salt - s. aureus is |
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diseases caused by s. epi and other coag neg staphs
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-bacterial endocarditis of prosthetic valve
-surgical prosthesis infection -bacteremia |
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disease caused by s. saprophyticus
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URINARY TRACT INFECTION
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6 extracellular substances produced by s. aureus
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exfoliative toxin (gets under epidermis)
leukocidin (kills WBC) enterotoxin (causes 1/3 of food poison) coagulase (fibrin walls off from immun) protein A (allows resist to phagocytos) others (v. virulent) |
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diseases of skin caused by s. aureus
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pimples/acne/folliculitis
carbuncles boils/furuncles impetigo cellulitis scalded skin syndrome |
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ritters disease
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caused by s. aureus; also known as scalded skin syndrome; in infants, bacteria under epidermis cleaves skin off
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diseases of digestive tract caused by s. aureus
what toxins? charact: length, symptoms, causes |
food poisoning
Enterotoxins A and D 2-6 hr incub., nausea/vomit/diahhr., unrefrig high protein foods self limiting |
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respiratory disease caused by s. aureus
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pneumoniae; yellow sputum
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tampons + s. aureus =
caused by |
toxic shock syndrome
tsst-1 initial - high fever, dizzy, vomit, shock 2 days later - sunburn rash, hypotens. |
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staph does what to bones
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causes osteomyelitis
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when is an epidemiological investigation conducted?
what is it |
outbreak;phage typing nad plasmid analysis
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phage typing
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a plate has different phages which infect various bacteria; if the same spot on two plates shows no growth, the bacteria are the same.
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what does s. aureus produce, and therefore you can't prescribe
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b-lactamase; penicillins or cephalosporins
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use to treat s. aureus: (simple)
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methicillin
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to treat MRSA
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vancomycin
its due to PBPs |
