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16 Cards in this Set
- Front
- Back
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Bugs that do NOT stain well
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These Rascals May Microscopically Lack Color
Treponema pallidum (too thin) Rickettsia (intracellular) Mycobacterium (high-lipid-content cell wall... acid fast... contains mycolic acid) Mycoplasma (no cell wall... cell membrane contains sterols) Legionella pheumophilia (primarily intracellular) Chlamydia (intracellular parasites... lacks muramic acid in cell wall) |
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Giemsa stains what bugs?
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Borrelia
Plasmodium trypanosomes Chlamydia |
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PAS (periodic acid-Schiff) stains what bugs?
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Stains glycogen and mucopolysaccharides
Used to diagnose Whipple's disease - Thopheryma whippelii Also - alpha1 antitrypsin in the liver, gaucher's disease, and ALL |
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Ziehl-Neelsen (carbol fushcin) stains what bugs?
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Acid-fast organisms:
Mycobacteria Nocardia |
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India ink stains what bugs?
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Cryptococcus neoformans (mucicarmine can also be used to stain thick polysaccharide capsule red)
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Silver stain stains what bugs?
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Fungi (Pneumocystis)
Legionella |
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What culture is required for H. influenzae?
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Chocolate agar with factors
V (NAD+) and X (hematin) |
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What culture is required for N. gonorrnea?
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Thayer-Martin (or VPN - vancomycin, polymyxin, nystatin) media
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What culture is required for B. pertussis?
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Bordet-Gengou (potato) agar
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What culture is required for C. diphtheriae?
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Tellurite plate
Loffler's media Methylene blue stain for granules |
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What culture is required for M. tuberculosis?
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Lowenstein-Jensen agar
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What culture is required for M. pneumoniae?
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Eaton's agar
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What culture is required for lactose-fermenting enterics?
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Pink colonies on MacKonkey's agar
Note: E. Coli is also grown on eosin-methylene blue (EMB) agar, as green colonies with metallic sheen |
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What culture is required for Legionella?
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Charcoal yeast extract agar buffered with cysteine and iron
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What culture is required for fungi?
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Sabouraud's agar
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What culture is required for Enterocci (faecalis & faecium)?
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Bile salts and 6.5% hypertonic saline
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