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69 Cards in this Set

  • Front
  • Back
What stains is this? What does it stain?
-Fontana Masson

-Melanin pigment in malignant melanoma
What stain is this? What does it stain?
-Alcian Blue

-goblet cells
What structure is stained blue?
-goblet cells
What is the name of this stain? What is this stain used for?
-Hematoxylin and Eosin (H and E)

-routine stain, nuclear detail
What is this stain? What does it stain?
-Toluidine Blue

-Mast Cells
What is the name of this stain? What does it stain?
-PAS

-carbohydrates, goblet cells
What are the bright magenta structures called? Why is it stained that way?
-goblet cells

-glycol groups are converted to aldehydes by the periodic acid which allows them to take up the Schiff's reagent
What stain is this? What does it stain?
-Masson Trichrome

-connective tissue fibers (elastic vs. collagenous)
What stain is this? What does it stain?
-Verhoeff-Van Gieson

-differentiates collagenous and elastic tissue
What structures are represented by the different arrows?
A: Muscle Cells

B: Elastic Fibers

C: Collagenous Fibers
What stain is this? What does it stain?
-Masson Verhoeff combination

-differentiates between collagenous and elastic fibers
What structures do the arrows point to?
A: Muscle Tissue

B: Collagen fibers

C: Elastic Fibers
What is the name of this stain? What does it stain?
-Mallory's Connective Tissue Stain

-connective tissue
What is represented by the red portion? What is represented by the blue portion?
-parenchymal tissue (secretory cells)

-CT stoma (supporting elements)
What is the name of this stain? What is it used for?
-Golgi Stain

-nerve cells and fibers
What is the name of this stain? What does it stain? What are the name of the dark purple/black spots and what is their significance?
-Cresyl Violet

-nervous tissue

-Nissil Bodies are stained dark because they contain more RNA which takes up the dye more readily
What is the name of this stain? What does it stain?
-Luxol Fast Blue

-myelin sheaths of axons and other nerve fibers
What is the name of this stain? What is it used to stain?
-Luxol Fast Blue-Cresyl Violet combination

-nerve fibers with more distinct detail than plain LFB
What is the name for this stain? What does it stain?
-Kluver-Barrera

-neurons
What are the names of the structures the arrows are pointing to?
What is the name of this stain? What does it stain for? What is represented by the black portion of the stain?
-Fontana Masson

-melanin pigment

-melanin pigment
What is the name of this stain? What is it used to stain?
-Prussian Blue

-iron pigment
What is the name of this stain? What is this stain used for?
-Toluidine Blue

-mast cells in tumors
What is the name of this stain? What does it stain?
-Osmium Tetroxide

-Lipids (in light microscopes)
What is the name of the stain on the left? Right? What are both of these stains staining?
-Oil Red O

-Sudan Black

-lipids (frozen sections)
What is the name of this stain? What does it stain?
-alcian blue

-mucins
What is responsible for the blue color in this stain?

What are the blue portions of this stain?
-copper

-mucins and mucosubstances
What structures are stained pink?
-cytoplasm
What is the name of this stain? What does it stain?
-alkaline phosphatase

-endothelial cells
What do the red portions of this stain signify?
-red portions show areas of alkaline phosphatase activity
What color will the nucleus stain in this stain?
-blue
What is the name of this stain? What does it stain?
-Bielschowsky stain

-neurofibrillary tangles and senile plaques
What is represented by the portion stained black?
-neurofibrillary tangles and senile plaques
What is the name of this stain? What is it used to stain?
-Cajal stain

-nervous tissue
What is the name of this stain? What does it stain for?
-Congo Red

-amyloid
What color represents a positive reaction?
-red

-the amyloid positive tissue stains red
What is the name of this stain? What does it stain for?
-Cresyl violet

-neurons and glia (nissl substances)
What does this stain bind to?
-acidic parts of the cells
What is the name of this stain? What does it stain?
-Fontana-Masson

-argentaffin granules and melanin
What is stained black? What is stained pink/red?
-argentaffin granules and melanin

-nucleus
What type of stain is this? (what family of stains)
-silver stain
What is the name of this stain? What does it stain?
-Giemsa stain

-peripheral blood smear and bone marrow
What is a secondary use for this stain?
-parasites and malaria
What reagent is responsible for the dark blue/purple color to this stain?
-methylene blue azure
What structures pick up the dark blue/purple color in this stain?
-acidic structures

-cytoplasm, chromatin, platelets, leukocytes
What is the name of this stain? What will it stain?
-Golgi stain

-neurons
What is the name of this stain?
-Hematoxylin and Eosin
What is the most common use for this stain?
-routine histology
What are the names of the dyes used in the stain? What does each stain?
-Hematoxylin stains nucleic acids (nucleus)

-Eosin stains cytoplasm, connective tissue, muscle, colloid, red blood cells, and decalcified bone matrix
What is the name of this stain? What does it stain?
-Luna stain

-elastin and mast cells
What is represented by the black portions of this stain? purple portion
-nucleus

-elastin and mast cells
What is the name of this stain? What is stained?
-Luxol fast blue

-myelin
What structures are stained blue? violet?
-myelin

-neurons
What is the name of this stain? What does it stain?
-Masson trichrome stain

-connective tissue
What are the three dyes that make up this stain?
-light green, iron hematoxylin, acid fuchsin
What is stained blue/green? red? pink? black?
-collagen fibers

-muscle and keratin

-cytoplasm

-nucleus
What is the name of this stain? What does it stain?
-Nissl stain

-nissl bodies in neurons (RNA and DNA)
What is stained purple/blue?
-nissl substances
What is the name for this stain? What does it stain
-Oil Red O

-lipids
What is stained by the red portion? blue/black portion?
-lipids

-nucleus
What is the name of this stain? What is it used to stain?
-osmium tetroxide

-lipids
What is represented by the brown portion? black portion?
-collagen and erythrocytes

-lipids and myelin
What is the name of this stain?
-pap stain
What is this stain used to stain?
-gynecological smears
-sputum
-urine
-cerebrospinal fluid
-abdominal fluid
-pleural fluid
-synovial fluid
-semminal fluid
-fine needle aspiration
What is the name of this stain? What does it stain?
-Periodic acid-Schiff

-glycogen, mucin
What is stained magenta? blue? pink?
-glycogen

-nuclei

-cytoplasm
What is the name of this stain? What does it stain?
-Prussian blue

-iron
What is the name of this stain? What is it used to stain?
-PTAH (phosophotungstic acid hematoxylin)

-striated muscle fibers and mitochondria
What is represented by the blue portion? red?
-muscle fibers and mitochondria

-collagen