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31 Cards in this Set
- Front
- Back
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types of mucopolysac
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1. neurtal: glands in GI tract, prostate, PAS +
2. Acid (simple or non-sulfated) epitheial cells cotain sialic acid PAS+, alicain blue at 2.5, colloidial iron, metachromatic, resist hyaluronidase 3. Acid (simple mesenchymal) contain hyaluronic acid tissue stroma, DO NOT STAIN WITH PAS, do stain with alcian blue at 2.5, colloidal iron, metachromatic dyes, no resit with hyaluronicacid, sarcomas 4. Acid (complex, sulfated, epithelia) adenocarcinomas, PAS +, alcian blue at 1, colloidial iorn, mucicarmine, metachromatic resist hyauronidase 5. Acid (complex, connective tissue) stroma, cartilate, bone chondrotin sulfate or kearatin PAS negative, alcian blue at 0.5 |
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colloidal iron "AMP" requires?
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formalin fixation
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colloidal iron blue color
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prussian blue
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mucicarmine stain uses? stains epithelial mucin?
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carmine (alum lake)
epithelial mucin red cryptococcus |
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mucin stain with most specificity?
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mucicarmine very SPECIFIC
VERY INSENSITIVE |
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most sensitive mucin stain?
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PAS
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colloidal iron predictable?
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no
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Mucin Stains
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PAS most sensitive
Mucicarmine most specific Alcian blue Colloidal Iron not predicatble |
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Masson's Trichrome stain
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nuclei deep blue
skeletal and smooth muscle red collagen and mucin light blue |
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Masson Trichrome 4 dyes used?
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hematoxylin
Biebrich scarlet acid fushsin aniline blue |
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Masson's Trichrome keratin and muscle fibers?
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red
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Masson's Trichroime collagen and bone
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blue or green
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Masson's Trichrome cytoplasm?
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light red or pink cytoplasm
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Masson's Trichrome cell nuclei?
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brown to black
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Weigert's iron hematoxylin?
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used in Masson's Trichrome
sequence of 3 solutions ferric chloride in diluted hydrochloric acid hematoxylin in 95% ethanol postassium ferricyanide solution alkalized by sodium borate used to stain nuclei blue |
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Solution A in Masson's Trichrome?
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plasma stain
acid fuchsin, Xylidine Ponceau, glacial acetic acid, and distilled water stains keratin and muscle red |
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Solution B Masson's Trichrome?
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phosphomolybdic acid in distilled water
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Solution C in Masson's Trichrome?
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fibre stain
light green SF yellowis or Fast Green FCF stains collagen i f blue then use methyl blue, water blue, ora niline blue can be used |
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Verhoeff-Van Gieson Stain?
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also called elastic-Van Gieson Stain (EVG)
elastic fibers blue-black to black collagen pale red other tissue yellow nuclei blue to black used to stain elatidc material vascular malfomations |
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only lipid stain that can be used on paraffin-embeded tissue
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Osmium tetroxide
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JXG stains
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CD68, lysozyme, HAM56, factor 13a
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xanthoma disseminatum stains
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CD68, lysozyme, factor 13a
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multicentric reticulohistiocytoma stains
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CD68, HAM56
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NXG
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lysozyme, CD68, CD11b
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SUPERFICIAL ACRAL FIBROMYXOMA STAINS
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CD34, CD10, VIMENTIN, FACTOR 13A (scattered cells)
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Mammary Paget's stains
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CK7+
GCDEFP, cathepsin B, EMA variable CEA CK20 neg |
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Primary extramammary Paget's stains
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S2 mammary paget's but GCDFP negative and more mucin (+mucicarmine, alcian blue 2.0, Hale's collodial iron, PAS)
CK7+ EMA+ cathepsin B + variable DEA |
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Secondary extrammamary Paget's
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assoc w internal malignancy
Lung carcinoma CK7+/CK20 neg Colon CK7-, CK20+ Bladder CK7+/CK20+ Prostate CK7-/CK20- |
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RENAL CELL CARCINOMA STAINS
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RCC-MA+
EMA+ CD10+ CAM 5.2+ CEA+ GLYCOGEN |
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hhf-35
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PAN-MUSCLE ACTIN
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SMOOTH MUSCLE STAINS
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TRICHROME RED
SMA MUSCLE SPECIFIC ACTIN PAN-MUSCLE ACTIN: HHF-35 DESMIN CALPONIN H-CALDESMON |
