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15 Cards in this Set

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Staining

Process of artificially coloring the organism with dyes/stains

Special staining

Use to demonstrate special features of the cell.

1.CAPSULAR STAINS

A.Hiss stain-Capsule is stained brown


B.Tyler stain-Capsule is stained light violet


C.Muir stain-Capsule is stained light blue


D.Negative/Indirectly/relief staining for capsule



a. India ink/ Nigrosin-Capsule is unstained; background is a black.


B.Congo red- Capsule is unstained; background I'd blue


C. Anthony's stain- Capsule is unstained; background is purple


2.Stains for Metachromatic Granules

A. Albert's stain- granules are block


B. Neisser stain- granules are dark blue


C Lindegran stain- granules are reddish brown


D. Leader's alkaline methylene blue stain (LAMB)- granules are dark blue


E. Burke's technique- modified gram's technique

3. Stain For Bipolar Bodies

A. Wayson stain- bipolar bodies are red

4.Stain For Flagella

A. Leifson stain- Flagella are red


B.Gray's stain

5. Stain For Endospore

A. Fulton-Schaeffer's technique- spores are green


B. Dorner stain- spores are red


C. Wirtz-Conklin stain- spores are green


6.Stain for Rickettsi and Chlamydia

A.For Rickettsia


A. Castaneda- blue


B.Machaivello- red


C.Giemsa- blue


B. For Chlamydia


A. Gimenez- inclusion bodies are red


B. Machiavello- red


C. Fiends- purple


7.7. Stain For Spirochetes

A. Fontana Tribondeau- spirochetes are dark brown or black



B. Levaditi Silver impregnation- spirochetes are block


C. India ink negative staining- spirochetes are unstained; black background

8. Stain for Mycoplasma

A. Dienes stain- blue

9. Stain For cell wall

A. Victoria blue dye- blue

10. Stains for Acid-Fast Bacilli

A.. Ziehl Neelsen ( Hot method)- AFB are red


B. Kinyoun ( Cold Method) - AFB are red


C. Truant's Fluorescence technique


D. Flickerson and kammer's Flourescence technique


E. Pappenheim's method- diffetentiates mycobacterium tuberculosis (red/ positive ) from mycobacterium smegmatis ( blue / negative )


F. Baumgarten's method- differentiates mycobacteriu m tuberculosis (red / negative)


G. Wadefite technique/ Fite Faraco technique- stain for mycbacterium leprae in tissue section; uses hematoxylin as secondary stain/ counterstain


11. Brown and Brenn Technique

Modified Gram's stain for bacteria section

12. Warthin-Starrysilver stain

Special staining for Bartonella, Helicobacter, Borrelia and Treponema

13. Hucker's modification of Gram's stain

For fungi and they are all gram positive