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15 Cards in this Set
- Front
- Back
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genome
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complete genetic material of an organism
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genomics
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sequencing, assembling, and analysing genomes
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whole genome sequencing identifies:
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genes, sequence, number, regulatory elements, non-protein coding RNA genes
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sanger sequencing
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ddNTPs terminate sequence early, run PCR and gel electrophoresis
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sanger pros
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accurate, long reads
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sanger cons
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expensive, low output
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whole genome sequencing/shotgun
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genome cut up, cloned + amplified, sequenced by computer
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illumina
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fix DNA onto bead + place in flow cell, add dNTPs with reversible blocking groups
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illumina pros
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accurate, cheap, high output
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illumina cons
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short reads
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oxford nanopore
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run DNA through alpha-hemolysin (bacterial transmembrane protein)
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oxford nanopore pros
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very long reads, cheap
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oxford nanopore cons
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high error rate
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SNPs as markers
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tend to be inherited with disease causing genes nearby
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genome wide association studies
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if 1 variant if more frequent at a locus it is considered associated
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