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70 Cards in this Set

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Identify Staphylococci
Gram (+), catalase (+)
Identify S. aureus
Gram (+), catalase (+), coagulase (+), beta-hemolytic, salt tolerant, ferments mannitol
Id non-S.aures
Gram (+), catalase (+), coagulase (-)
ID streptococci/enterococci
Gram (+), catalase (-)
ID streptococci
Gram (+), catalase (-) in pairs or chains
Culture of staph
Gram (+), grape like clusters
culture of strep
Gram (+), pairs or chains
culture of Enterococci
Gram (+) pairs or chains, resistant to bile salts
alpha -hemolytic, Gram (+), catalase (-)
Strep pneumoniae, Viridans strep
beta -hemolytic, Gram (+), catalase (-)
Strep pyogenes
non -hemolytic, Gram (+), catalase (-)
Group D strep
Group D Lancefield, Gram (+), catalase (-)
Enterococci
Gram (+) spore-forming, aerobic
Bacillus
Gram (+) spore-forming, anaerobic
Clostridium
location of Bacullus
soil and water (makes spores)
location of Clostridium
soil, water, and GI tract (makes spores)
Gram (+) spore forming rods
Bacillus, Clostridium
Gram (+) non-spore forming rods (5)
Actinomyces, Corynebacterium, Listeria, Lactobacillus, Nocardia
Gram (+) anaaerobic non-spore forming rod (1)
actinomyces
Gram (+) non-spore forming aerobic rods
Corynebacterium, Listeria, Lactobacillus, Nocardia
ID Corynebacterium
Gram (+) non-spore forming aerobic rod, club shaped or "chinese letters"
ID Listeria
Gram (+) non-spore forming aerobic rod, growth at 4 degrees C, tumbling motility in broth, facultative intracellular organism
ID Lactobacillus
Gram (+) non-spore forming aerobic rod, part of normal flora of mouth/vagaina
ID Nocardia
Gram (+) non-spore forming aerobic rod, filamentous, found in soil and water, is opportunistic, weakly acid fast
ID actinomyces
Gram (+) anaaerobic non-spore forming rod, filamentous and branching, non-acid fast
Gram (-) Cocci
Neisseria, Acinetobacter, Moraxella
ID Neisseria
Often in PMNs, grows best in chocolate agar w/ 5% CO2, or even better on Thayer-Martin agar. Oxidase (+), Ferments glucose
Gram (-) rods
a lot… 2 main groups are Enterobacteriaceae and non-enterbacteriaceae
Enterobacteriaceae
Gram (-) rods, ferment glucose, oxidase (-), normal habitat is GI
Lactose-fermenting enterbacteriaceae (4 groups) and ID
Gram (-) rods: Serratia, Enterobacter, Escherichia, Klebsiella. So pink/red on MacConkey agar
Non-lactose fermenting enterbacteriaceae (4 groups) and ID
Gram (-) rods: Proteus, Salmoella, Shigella, Yersinia. Clear on MacConkey
Proteus spp.
Non-lactose fermenting enterbacteriaceae, H2S producing, urease (+), swarming motility so makes "tree rinigs" in agar, common in UTI
Salmonella spp.
Non-lactose fermenting enterbacteriaceae, H2S producing, motile. Causes gastroenteritis
Shigella spp.
Non-lactose fermenting enterbacteriaceae, non-H2S producing, non-motile. LOW infectious dose. Can cause gasteroenteritis
Yersinia spp.
Non-lactose fermenting enterbacteriaceae, non-H2S producing, safety pin appearance. Can cause gastroenteritis, plague
Gram (-) rod with safey-pin appearance
Yersinia spp.
Non-enterobacteriacae groups (4)
Curved/spiral rods, opportunist, resp tract pathogens, zoonses
Curved/spiral rods that are Non-enterobacteriacae
Campylobacter spp, Vibrio spp, Helicobacter spp.
Successful opportunist that is Non-enterobacteriacae
Pseudomonas spp
pathogens of resp tract that are Non-enterobacteriacae
Haemophilus spp, Bordetella spp, Legionella spp.
Zoonses that are Non-enterobacteriacae
Pasturella spp, Brucella spp, Francisella spp, Bartonella spp
ID Campylobacter
"gull shaped wings", incubates at 42 degrees C, oxidase (+)
"gull shaped wings" bact that grows at 42 degree C
Campylobacter spp.
Campylobacter
Curved/spiral rods that are Non-enterobacteriacae
ID Vibrio spp.
Comma shaped, oxidase (+), Gram (-) rods
ID helicobacter spp.
Urease (+) microaerophile, Gram (-) rods
Pseudomonas spp.
oxidase (+), Gram (-) rod, can not use glucose
Haemophilus spp.
Gram (-) rod, fastidious, and requires Factor X and V
N. gonorrhoae vs. N. meningitides
N. gonorrhoeae only ferments glu, N. meningitides ferments both glucose and MALTOSE
Acinetobacter
Gram (-) bacilli, found in soil and water, can colonize the skin
Moraxella
Gram (-) bacilli, part of normal resp flora, NOT in inanimate environment. Can cause otitis media, bacteremia, meningitis, and others
Bordella spp.
Gram (-) rod, that grows in chain/pairs
Legionella
Gram (-) rod, stains poorly, use BCYE agar (buffered charcoal Yeast agar)
Pasturella spp.
Gram (-) rod, zoonotic, with bipolar staining
Brucella spp.
Gram (-) rod, zoonotic, small pleomorphic rod, slow growing
Francisella spp.
Gram (-) rod, zoonotic, requires cystiene for growth, occupational risk with animals
Bartonella spp.
Gram (-) rod, zoonotic, slow growing and hard to isolate. "cat scratch fever"
Spirochetes general discription
helical shaped, endoflagella, poor staining with Gram (-) wall
Spirochete bact (2)
Treponema spp and Borrelia sp
Treponema spp
spirochete, never been cultured
Borrelia spp.
Gram (-) spirochete, arthropod borne, Lyme disease
Obligate intracellular Gram (-) bact (6)
Chlamydia, Chlamydiophila, Rickettsia, Ehrlichia, Anaplasma, Coxiella
Obligate intracellular Gram (-) description
must be in eukaryotic cells to grow and multiply, stain poorly but have Gram (-) wall, cannot culture on semi-solid media
Chlamydia and Chlamydiphila
intracellular, so Gram does not work, steal ATP/NAD from host
Rickettsia
arthropod-borne, obiligate intracellular
Ehrlichia spp
arthropod-borne and infects leukocytes, obiligate intracellular
Anaplasma spp
arthropod-borne and infects leukocytes, obiligate intracellular
Coxiella spp
Zoonitic, obiligate intracellular
Mycobacteria spp
small straight rods with poor Gram stain, are acid fast. Have mycolic acids in cell walls. Slow growing, resistant to drying, chemical agents, germicides. TB and lepracy
Mycoplasma spp
lack cell wall, pleomorphic, sensitive to environmental conditions, hard to grow, fried egg appearance, normal flora, but can cause pneumonia or GU infections