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70 Cards in this Set
- Front
- Back
Identify Staphylococci
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Gram (+), catalase (+)
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Identify S. aureus
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Gram (+), catalase (+), coagulase (+), beta-hemolytic, salt tolerant, ferments mannitol
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Id non-S.aures
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Gram (+), catalase (+), coagulase (-)
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ID streptococci/enterococci
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Gram (+), catalase (-)
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ID streptococci
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Gram (+), catalase (-) in pairs or chains
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Culture of staph
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Gram (+), grape like clusters
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culture of strep
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Gram (+), pairs or chains
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culture of Enterococci
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Gram (+) pairs or chains, resistant to bile salts
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alpha -hemolytic, Gram (+), catalase (-)
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Strep pneumoniae, Viridans strep
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beta -hemolytic, Gram (+), catalase (-)
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Strep pyogenes
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non -hemolytic, Gram (+), catalase (-)
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Group D strep
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Group D Lancefield, Gram (+), catalase (-)
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Enterococci
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Gram (+) spore-forming, aerobic
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Bacillus
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Gram (+) spore-forming, anaerobic
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Clostridium
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location of Bacullus
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soil and water (makes spores)
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location of Clostridium
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soil, water, and GI tract (makes spores)
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Gram (+) spore forming rods
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Bacillus, Clostridium
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Gram (+) non-spore forming rods (5)
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Actinomyces, Corynebacterium, Listeria, Lactobacillus, Nocardia
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Gram (+) anaaerobic non-spore forming rod (1)
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actinomyces
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Gram (+) non-spore forming aerobic rods
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Corynebacterium, Listeria, Lactobacillus, Nocardia
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ID Corynebacterium
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Gram (+) non-spore forming aerobic rod, club shaped or "chinese letters"
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ID Listeria
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Gram (+) non-spore forming aerobic rod, growth at 4 degrees C, tumbling motility in broth, facultative intracellular organism
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ID Lactobacillus
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Gram (+) non-spore forming aerobic rod, part of normal flora of mouth/vagaina
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ID Nocardia
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Gram (+) non-spore forming aerobic rod, filamentous, found in soil and water, is opportunistic, weakly acid fast
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ID actinomyces
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Gram (+) anaaerobic non-spore forming rod, filamentous and branching, non-acid fast
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Gram (-) Cocci
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Neisseria, Acinetobacter, Moraxella
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ID Neisseria
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Often in PMNs, grows best in chocolate agar w/ 5% CO2, or even better on Thayer-Martin agar. Oxidase (+), Ferments glucose
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Gram (-) rods
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a lot… 2 main groups are Enterobacteriaceae and non-enterbacteriaceae
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Enterobacteriaceae
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Gram (-) rods, ferment glucose, oxidase (-), normal habitat is GI
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Lactose-fermenting enterbacteriaceae (4 groups) and ID
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Gram (-) rods: Serratia, Enterobacter, Escherichia, Klebsiella. So pink/red on MacConkey agar
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Non-lactose fermenting enterbacteriaceae (4 groups) and ID
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Gram (-) rods: Proteus, Salmoella, Shigella, Yersinia. Clear on MacConkey
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Proteus spp.
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Non-lactose fermenting enterbacteriaceae, H2S producing, urease (+), swarming motility so makes "tree rinigs" in agar, common in UTI
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Salmonella spp.
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Non-lactose fermenting enterbacteriaceae, H2S producing, motile. Causes gastroenteritis
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Shigella spp.
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Non-lactose fermenting enterbacteriaceae, non-H2S producing, non-motile. LOW infectious dose. Can cause gasteroenteritis
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Yersinia spp.
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Non-lactose fermenting enterbacteriaceae, non-H2S producing, safety pin appearance. Can cause gastroenteritis, plague
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Gram (-) rod with safey-pin appearance
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Yersinia spp.
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Non-enterobacteriacae groups (4)
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Curved/spiral rods, opportunist, resp tract pathogens, zoonses
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Curved/spiral rods that are Non-enterobacteriacae
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Campylobacter spp, Vibrio spp, Helicobacter spp.
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Successful opportunist that is Non-enterobacteriacae
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Pseudomonas spp
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pathogens of resp tract that are Non-enterobacteriacae
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Haemophilus spp, Bordetella spp, Legionella spp.
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Zoonses that are Non-enterobacteriacae
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Pasturella spp, Brucella spp, Francisella spp, Bartonella spp
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ID Campylobacter
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"gull shaped wings", incubates at 42 degrees C, oxidase (+)
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"gull shaped wings" bact that grows at 42 degree C
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Campylobacter spp.
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Campylobacter
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Curved/spiral rods that are Non-enterobacteriacae
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ID Vibrio spp.
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Comma shaped, oxidase (+), Gram (-) rods
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ID helicobacter spp.
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Urease (+) microaerophile, Gram (-) rods
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Pseudomonas spp.
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oxidase (+), Gram (-) rod, can not use glucose
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Haemophilus spp.
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Gram (-) rod, fastidious, and requires Factor X and V
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N. gonorrhoae vs. N. meningitides
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N. gonorrhoeae only ferments glu, N. meningitides ferments both glucose and MALTOSE
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Acinetobacter
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Gram (-) bacilli, found in soil and water, can colonize the skin
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Moraxella
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Gram (-) bacilli, part of normal resp flora, NOT in inanimate environment. Can cause otitis media, bacteremia, meningitis, and others
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Bordella spp.
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Gram (-) rod, that grows in chain/pairs
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Legionella
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Gram (-) rod, stains poorly, use BCYE agar (buffered charcoal Yeast agar)
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Pasturella spp.
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Gram (-) rod, zoonotic, with bipolar staining
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Brucella spp.
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Gram (-) rod, zoonotic, small pleomorphic rod, slow growing
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Francisella spp.
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Gram (-) rod, zoonotic, requires cystiene for growth, occupational risk with animals
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Bartonella spp.
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Gram (-) rod, zoonotic, slow growing and hard to isolate. "cat scratch fever"
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Spirochetes general discription
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helical shaped, endoflagella, poor staining with Gram (-) wall
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Spirochete bact (2)
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Treponema spp and Borrelia sp
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Treponema spp
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spirochete, never been cultured
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Borrelia spp.
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Gram (-) spirochete, arthropod borne, Lyme disease
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Obligate intracellular Gram (-) bact (6)
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Chlamydia, Chlamydiophila, Rickettsia, Ehrlichia, Anaplasma, Coxiella
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Obligate intracellular Gram (-) description
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must be in eukaryotic cells to grow and multiply, stain poorly but have Gram (-) wall, cannot culture on semi-solid media
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Chlamydia and Chlamydiphila
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intracellular, so Gram does not work, steal ATP/NAD from host
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Rickettsia
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arthropod-borne, obiligate intracellular
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Ehrlichia spp
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arthropod-borne and infects leukocytes, obiligate intracellular
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Anaplasma spp
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arthropod-borne and infects leukocytes, obiligate intracellular
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Coxiella spp
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Zoonitic, obiligate intracellular
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Mycobacteria spp
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small straight rods with poor Gram stain, are acid fast. Have mycolic acids in cell walls. Slow growing, resistant to drying, chemical agents, germicides. TB and lepracy
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Mycoplasma spp
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lack cell wall, pleomorphic, sensitive to environmental conditions, hard to grow, fried egg appearance, normal flora, but can cause pneumonia or GU infections
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