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8 Cards in this Set
- Front
- Back
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Endocytic Pathway
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plasma membrane
endosome lysosome |
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Pulse Chase Experiment
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labelling with leucine C14 for 3 minutes - 3H-Leucine
incubated in chase medium for varying times After labelling: 3 minutes - ER 7 minutes - golgi 37 minutes - vacuoles 117 minutes - zymogen granules |
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ER
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modifications
folding N linked glycosylation assembly quality control (proteasome destruction) |
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Golgi
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final modifications
packaging distribution - secretory granules, cell surface, endosomes, lysosomes |
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Categories of Protein Transport
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1. Transmembrane transport: acceptor has membrane
2. Gated transport: into nucleus from pores 3. Direct connections: between Golgi 4. Protein detaches and diffuses through cytoplasm 5. Vesicular transport |
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Vesicular Transport
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Step 1: sorting, budding, separation
Step 2: transport Step 3: storage in some cases (synaptic vesicles) Step 4: recognition of target and fusion |
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Tubular Transport Intermediate
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Golgi to ER
Golgi to cell membrane |
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COP2 Vesicles
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Sec12 GEF
Activates SAR1-GDP to SAR1-GTP Active form gets locked in the membrane Recruits inner COP2 subunits: Sec23/Sec24 - Recognizes the cargo Bind outer COP2 subunits: Sec13/Sec31 - Membrane curvature and budding Sar1 hydrolyzes GTP - uncoating of vesicle Fusion with VTC |
