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20 Cards in this Set
- Front
- Back
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furuncle |
Staph aureus infected hair folicle |
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carbuncle |
Staph aureus infection deep under the skin |
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MRSA |
Methicillin resistant Staph aureus
- Mec A gene associated with MRSA |
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VISA |
Vancomycin intermediate Staph aureus
- used to be drug of choice
- first noted in Japan in 1996 |
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VRSA |
Vancomycin resistant Staph aureus
- first seen in 2002 in Michigan, Penn., New York
- screen for by putting on Brain Heart Infusion agar with 6 ug/ml of vancomycin (grows = pos.) |
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Epidermolytic toxin |
-toxin produced by Staph. aureus
-scalded skin syndrome
-most common in infants |
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Cytolytic toxins |
alpha-hemolysin--destroys platelets
beta-hemolysin-- destroys RBC's
gamma-hemolysin--destroys tissue and WBC's |
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Drugs used to treat MRSA |
Rifampin ---these 2 best used together to treat-- Bactrum
Vancomycin |
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Micrococcus leutus on BAP agar |
bright yellow |
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Micrococcus leutus gram stain |
gram +
cocci
tetrads |
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Micrococcaceae family characteristics |
-gram + cocci, tetrads - aerobic or facultative - nonfafstidious - nonmotile (no pili or flagella) - catalase + - will reduce nitrates to nitrites - grows on CNA & PEA agar (grows gram +) |
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Catalase reagent/test reaction |
3% H2O2 (hydrogen peroxide)
+ bubbles - no bubbles
**All are positive |
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Coagulase reagent/test reaction |
Rabbit plasma
+ clotting - no clotting
**Staph aureus + |
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Glucose reagent/test reaction |
10% glucose bromthymol blue
oxidizer = top yellow fermenter = bottom or all yellow asaccralytic = no yellow
**Staphs are Fermenters **Micro is Asaccralytic |
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Lysostaphin reagent/test reaction |
lysostaphin
+ clear (not lysed) - cloudy (lysed)
**Staph aureus + |
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Microdase disk reagent/test reaction |
6% tetramethyl-p-phenylenediamine dihydrochloride
+ blue - no color
**Micro + |
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"A" disk (Bacitracin) reagent/test reaction |
.04 units Bacitracin
Sensitive > or = 10mm Resistant < 10mm
**Micro Sensitive |
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Furzolidone disk reagent/test reaction |
Furzolidone
Sensitive > or = 15mm Resistant < 15 mm
**Micro Resistant |
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Novobiocin disk reagent/test reaction |
Novobiocin
Sensitive > 16mm Resistant < or = 16mm
**Staph saprophyticus Resistant **Staph epidermidis Sensitive |
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Mannitol (MSA agar) reagent/test reaction |
Mannitol 7.5% NaCl (sodium chloride)
+ pink colonies (ferments mannitol) - yellow colonies (does not ferment mannitol)
**Staph epidermidis yellow - **Micro no growth |
