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24 Cards in this Set
- Front
- Back
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STAINING
- Gram stain is weak & limiting for these bugs (x7) |
(Understand These Microbials May Really Lack Color)
- Ureaplasma Urealyticum - Treponema - Mycobacterium - Mycoplasma - Rickettsia - Listeria - Chlamydia |
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STAINING
- Giemsa staining bugs (x4) |
- Borrelia
- Chlamydia - TRYPANOSOMES - Plasmodium |
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STAINING
- PAS (periodic acid Schiff) stains what structures? - PAS is used to Diagnose what Dz? |
- Glycogen
- Mucopolysaccharides - Whipple's Disease (Tropheryma whippleii) |
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STAINING
- Acid Fast organisms (e.g.-mycobacterium) are stained with this |
Ziehl-Neelson
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STAINING
- Cryptococcus Neoformans can be stained with? |
- India ink
(mucicarmine can also be used to stain thick polysaccharide capsules red) |
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STAINING
- Silver staining bugs |
- Legionella
- Fungi (e.g.- pneumocystis) |
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CULTURE
- H. influenzae? |
Chocolate agar
(with factors V & X) |
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CULTURE
- N. gonorrhoeae? |
Thayer Martin (or VPN) media
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CULTURE
- B. pertussis? |
(BORDETella, use BORDET-gengou)
- Bordet-Gengou (potato) agar |
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CULTURE
- C. diphtheriae? |
(Corny jokes, Tell Her Right, and she'll Loff!"
- Tellurite agar - Loffler's media |
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CULTURE
- Mycobacterium Tuberculosis? |
(TB = LJ)
- Lowenstein-Jensen agar |
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CULTURE
- Mycoplasma Pneumoniae? |
- Eaton's agar
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CULTURE
- Lactose Fermenting Enterics? |
- MacConkey's agar
(shows up pink colonies) |
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CULTURE
- Besides MacConkey's agar, E. coli can also be grown on? |
- Eosin Methylene Blue (EMB) agar
(shows up as blue-black colonies w/ metallic sheen) (EMB = E.coli Materializes Blue/Black) |
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CULTURE
- Legionella? |
Charcoal Yeast Extract agar
buffered with Cysteine |
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CULTURE
- Fungi? |
- Sabouraud's agar
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CULTURE
- VPN agar for what organism? - VPN agar AKA? - what does VPN stands for? (what does each inhibit?) |
- H. influenzae
- Thayer-Martin - Vancomysin (inhibits GPs) - Polymyxin (inhibits GNs) - Nystatin (inhibits Fungi) |
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CULTURE
- Legionella agar is CYE which is reinforced with? |
- Cysteine
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CULTURE
- H. influenzae agar is Chocolate agar, with what factors added? |
Factors V (NAD+)
Factors X (Hematin) |
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LEGIONELLA
- stain? - culture? |
- Silver stain
- CYE reinforced with Cysteine |
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CHLAMYDIA
- Chlamydia weakly staining? - Chlamydia strongly staining? - Chlamydia is intracellular or extracellular? - Chlamydia lacks what component in cell wall? |
- weak Gram staining
- Giemsa stain (same as Borrelia) - Intracellular (same as rickettsiae & legionella) ("Really Lacks Color") - Muramic acid (is lacking in cell wall) |
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CULTURE
- Vibrio cholera? |
- T CBS
(Thiosulfate Citrate Bile-salt Sucrose) |
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CULTURE
- Anaerobes? |
- Thioglycate
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CULTURE
- which bacteria likes Alkaline growth media for growth? |
- Vibrio cholera
(maybe b/c its from the sea?) |
