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34 Cards in this Set
- Front
- Back
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What type of RNA is Polymerase II responsible for making?
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mRNA
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What does mRNA do?
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Encodes the polypeptide sequence
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What type of RNA is Polymerase III responsible for making?
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tRNA
snRNA 5S rRNA |
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what do tRNAs do?
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AA carriers
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whadt do snRNAs do?
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splice
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What are 5S rRNAs?
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Ribsomes
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What type of RNA is Polymerase I responsible for making?
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rRNA
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what does rRNA do?
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Components of ribosomes
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What is the most abundant RNA in our bodies?
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rRNA=90%
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What is least abundant RNA?
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mRNA
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Is upstream 5' or 3' and is it positive or negative?
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5'
negative |
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Is downstream 5' or 3' and is it positive or negative?
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3'
positive |
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is the promoter upstream of the gene or downstream?
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upstream
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what is the promoter?
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Region of DNA upstream of the gene
used to activate or repress Txs of gene |
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what is the minimial promoter?
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A region that will drive low level constituative expression. Its always on, always on low level
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What is the Enhancer?
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Located a distance away from the gene
- its orientation can be flipped!! |
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Where can you find the TATA box?
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in the minimal promoter
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What is the consensus seq for TATA ox?
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TATA t/a A
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What is TBP?
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TATA binding protein
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What is the initiate complex composed of?
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TBP + general Txs Factors at the MINIMAL PROMOTEr
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What is a recognition sequence?
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A consensus sequence which will be bound by a TF
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What is the 2nd most common Recognition Sequence? What does it do?
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CCAAT box
Raised baseline Txs |
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What is the most common recognition sequence?
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TATA box
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What is the DNA binding domain and what does it do?
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region of a Txs factor that recognizes and binds a specific sequence of DNA
- they have a + charge and this form Ionic bonds with - phosphates of DNA |
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What is the activation Domain?
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- Rgion of Txs Factor that INDUCES RNA SYNTHESIS via Attracting RNA polymerase
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What is the Acid Blob Model?
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The Activation Domain is negativly charges and attratct Pol non-specfically
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What observations are the Acid BLob Model based on?
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The Activation Domain is:
- most variable region of gene - have random coils, as opposed to specfic structures - attract the same RNA pols, no specificity - have - charge to attract RNA pol |
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What are the 2 domains of TF? what are their charges?
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DNA binding Domain (+)
Activation Domain (-) |
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What are the 4 types or Txs factors?
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Helix-loop-helix
Helix-turn-helix Zinc Fingers Luecine Zippers |
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What makes TF families, a family?
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They have identical DNA binding Domain sequences
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What is an example of the HTH family?
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Hox genes: aid in segmentation
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how do Zinc fingers bind? and what is important about it binding domain?
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they bind in the major loops of the DNA helix
- the fingers are made up of histidine and cystein residues chelated to a central zing ion, creating the loops |
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What is the imnportance of the HLH DNA binding domain?
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have 2 a helices sep by a linker region
- HLH factors (+,-, ubiquitous) form heterodimers |
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what is the importance of Leucins zippers?
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- they have luecines every 7 AA
- Leucine=HYDROPHOBIC - they have a scissor grip on the DNA |
