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98 Cards in this Set
- Front
- Back
What are genes made of?
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the nucleotides ATCG
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What are chromosomes made of?
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DNA
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What is the gene code for proteins?
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polypeptides
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What do proteins control?
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cells and the organism comprised of those cells
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What makes up the cell structure?
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proteins
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What is the hypothesis for one gene-one protein?
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each gene codes for one protein (might not be true since genes work together)
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What is protein synthesis?
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Deciphering the DNA code for proteins.
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Where does the assembly of protein take place?
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In the ribosome.
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Where is the ribosome located?
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In the cytoplasm of the cell, away from the nucleus and DNA
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What is a ribosome made up of?
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A small subunit and a large subunit
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What does tRNA make up the structure of?
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the ribosome
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What are the large and small subunits made of?
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Tangled masses of tRNA
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What are the three parts of an RNA nucleotide?
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ribose (a sugar), a phosphate group, a nitrogenous base
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How many strands does RNA have?
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one
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How many strands does DNA have?
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two
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What are the four bases of RNA?
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A,C, G, U
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What sugar is in RNA?
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ribose
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What are the four bases of DNA?
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A,T,C, G
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What sugar is in DNA?
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deoxyribose (lacks an oxygen)
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What process makes mRNA?
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transcription
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When does transcription begin?
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Only when a cell needs a protein.
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How many proteins does DNA have the code for?
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thousands
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How much of the DNA is used at one time to make a needed protein?
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Only the part with the code for that protein.
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What is made when proteins that are not needed are transcribed anyway?
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mutations and defects
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What does it men if a gene that codes for something is turned on?
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It starts making the protein
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What is the first step to transcription?
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The hydrogen bonds between the DNA bases are broken.
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What breaks the hydrogen bonds to make mRNA?
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the enzyme used to match the nucleotides - polymerase.
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What is the second step in transcription?
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The enzyme polymerase matches free floating RNA nucleotides in the nucleus to the separated molecule.
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What is the third thing that happens in transcription?
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A specific sequence of DNA marks the initiation and termination of the gene that is going to be made into a protein.
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What is the fourth thing that happens in transcription?
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The polymerase makes a complimentary strand to the parent strand that is RNA.
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What is RNA elongation?
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The process of making mRNA.
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What is the order of primes when making RNA?
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The RNA polymerase makes RNA from the 5' end to the 3' end.
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What site on RNA does the second tRNA bond to?
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the A site
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What is the first part of the DNA sequence called?
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the promoter
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What is the end part of the DNA called?
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the terminator
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What is the relationship between the promoter and RNA polymerase?
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RNA polymerase starts RNA creation at the promoter.
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What happens to the DNA molecule as the RNA polymerase moves along it?
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The hydrogen bonds split, and the mRNA is separated.
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How can you tell that a molecule is RNA?
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It has a single strand and uracil bases.
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What is an anticodon?
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A sequence of three nucleotides in transfer RNA that binds to the complementary triplet (codon) in messenger RNA to specify an amino acid during protein synthesis.
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What bonds with each anticodon letter?
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A-U
U-A G-C |
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What site on the ribosome does the tRNA bond to?
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the P site
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What site on the ribosome does the second tRNA bond to?
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the A site
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What happens to the amino acids in translation?
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the P site's tRNA releases amino acid and the A bonds to the other amino acid of A site's tRNA
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What does RF stand for?
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release factor
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What does GUU stand for?
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valine
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What does GAA stand for?
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Glutamic acid
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What is UAA?
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stop
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How many codons are there?
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64
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How many needed amino acids are there?
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20
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What are start and stop codons?
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those at the beginning and end of an mRNA strands.
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What is the start codon?
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AUG
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What are the stop codons?
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UAA, UAG, UGA
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AUG?
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Methionine
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UAA?
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stop
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UUA
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lencine
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CGU
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arginine
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UGU
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cysteine
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CUU
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leucine
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AGG
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arginine
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AAA
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lysine
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CAA
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glutamine
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GAG
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glutamic acid
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GCC
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alanine
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AAC
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asparagine
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CCC
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proline
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UUU
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phenylalanine
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GGA
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glycine
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UGG
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tryptophan
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What amino acid must every protein start with?
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methionine
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What must every protein end with?
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stop
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methionine
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AUG
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asparagine
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AAU
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threanine
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ACG
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stop
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UAA
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leucine
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CUG
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proline
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GGG
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leucine
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CUA
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If a protein does not end with a stop, what is it?
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a mutation
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What do the nucleotides A,T, C and G make up
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genes
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What do genes code for?
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proteins
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What is protein synthesis?
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The process through which DNA codes are deciphered.
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How many sub units make up the ribosome?
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2 sub units - the small and the large
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Where is the ribosome located?
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In the cytoplasm of the cell, away from the nucleus and the DNA
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Where in the cell is the DNA?
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The DNA is in the nucleus.
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How does the DNA relay its instructions to the ribosome?
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through the RNA (ribonucleic acid)
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What are the three types of RNA
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transfer, ribosomal, and messenger
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What does mRNA do?
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mRNA carries the message from the DNA in the nucleus to the the ribosome in the cytoplasm.
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Which RNA carries the amino acids to the ribosome to make the proteins?
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transfer RNA (tRNA)
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Amino acids are what of the proteins?
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monomers
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During transcription, what is the template for the mRNA?
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the DNA in the nucleus
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What are the monomers of the proteins?
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amino acids
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The instructions in mRNA are in the form of?
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codons
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How many bases are in a codon code?
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3
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How many amino acids are there?
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20
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What DNA strand does this mRNA come from ' AUG UUC GAA GCU 3'
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3' TAC AAG CTT CGA 5'
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When do multiple ribosomes work on the same mRNA to translate it together?
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when it is very long
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Give a summary of transcription:
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A) DNA unzips
B) RNA polymerase subunits attach to the mRNA C) RNA polymerase attaches free floating RNA nucleotides to the DNA template D) Enzymes cut out the introns and splice together the exons E) mRNA is made and it leaves the nucleus into the cytoplasm. |
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Give a summary of translation:
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F) ribosomal subunits attached to the mRNA
G) the first tRNA carries amino acid tothe ribosome and bonds at the P site H) the anticodon on the tRNA matches to the codon on the mRNA I) the next tRNA bonds to the mRNA at the A site J) the tRNAs release the amino acid K) the amino acid bonds together and forms a polypeptide protein |