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41 Cards in this Set
- Front
- Back
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What kindof pathogen is in the tribe Ricketssia, and what genuses does it entail?
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Obligate intracellular parasites, Ricketssia and Rochalimea, not Coxiella
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What are Ricketssieae trasnmitted by?
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Arthropod vectors - fleas, ticks mites
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Treatment of Rickettsieae?
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Tetracycline/Deoxycycline
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Short rods, barely visible under the light microscope, stains poorly with gram stain (stain with Giemsa/polychromatic stain, reproduce by binary fission, small rods, cocobacillus
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Rickettsias
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Which is the only microne in Family Ricketssia that can reproduce outside of the cell?
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Rochalimaea quintana
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Characteristics of metabolism in Rickettsia?
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glutamate oxidation with phosphorylation. Cell wall leaks enzymes and ATP, membranes are unusually permeable allows use of enzymes/molecules from the host cell.
Slow grower |
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Pathogenicity of Ricketssiaceae?
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Growth in endothelial cells of small blood vessels. Hyperplasia of those cells with Thrombus formation, obstruction of blood flow and petechial rash. Rickettsiae found in macrophages near vascular lesions
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How do rickettsieaceae exit from cells
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R. prowazekii - cell lysis
R. rickettsia - extruded from the cell O. tsutsugamushi - exits via buds |
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True or false: Basis of pathogenicity in Rickettsiaceae is shown to be endotoxin, exotoxin, and cytotoxic enzymes
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False, no toxins in rickettsia
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What microbe is transmitted through aerosol?
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C. burnetii
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Lab Identification of Rickettsia
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Weil-Felix Reaction
- antibodies formed during infection cross react with polysaccharide O antigens of certain Proteus strains, OX, OX-2, OX-19, OX-K |
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What weil-Felix reactions does typhus have?
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OX-19 +4
OX-2 + OX-K 0 |
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What weil-felix reactions does spotteed fever have?
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OX-19 +4|+
OX-2 + | +4 OX-K 0 | 0 |
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What Weil-Felix reactions does Scrub Typhus have?
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OX-19 0
OX-2 0 OX-K 0 |
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What Weil-felix reactions does Q fever have
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All 0
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What is the source of OX antigen?
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Proteus vulgaricus
Weil-Felix: 70 - 90% sensitivity |
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Lab ID using animal inoculation
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Male Guinea Pigs
- susceptible to R. prowazekii, R. typhi, R. rickettsii. - refractive to O. tsutsugamushi typhi/rickettsii cause scrotal swelling |
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How is a Giemsa stain performed?
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smear is air dried, fixed with absolute methanol (5 min.), air dried. Covered with giemsa stain for 1 hr. rinsed with 95% ethanol, dry.
Rickettsia - blue Nucleus - Pink |
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Collection of specimens of Rickettsia
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Blood or tissue sample quickly frozen at - 70 C,
direct immunoflouresence |
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Where can Rickettsia be isolated from?
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small lab animals, embryonated eggs.
Coxiella - has been isolated in human lung fibroblasts |
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What is used to differ between diseases due to Rickettsiae
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- which vector
- Weil-felix reaction - intracellular location of organism, cytoplasm, nucleus - distribution of Rash - results of animal inoculation |
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What does typhus cause
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Delirium/Stupor
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Transmitted by body louse defecation into broken skin, +4 OX19, + OX2, 0 OXK, bacteria located in cytoplasm, rash on trunk and spreads to extermities, inoculation of Guinea Pigs results in mild disease/rise in body temp.
Disease/Microbe/Treatment? |
Epidemic typhus
R. prowazekii Tetracycline |
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Symptoms of epidemic typhus?
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Rash on trunk spreads to extermities.
1-2 weeks post bite -chills, fever, headache, exhaustion, body temp 40C for 2-3 days, rash days 4-7. Becomes hemorrhagic, causes stupor/delirium |
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Secondary disease to epidemic typhus?
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Brill-zinsser Disease (latent form typhus)
R. prowazekii - no rash, antibodies appear earlier, no OX19 reaction, louse maybe be present |
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Arthropod - rat flea
Mammal - rodents rash on trunk, spreads to extremities Guinea Pig - testicle lesions swelling of scrotum Weil-Felix - +4 OX19, + OX2, 0 OXK BActeria locataed in cytoplasm of cells Disease/microbe/treatment |
Endemic typhus
R. typhi Resolves in 3 weeks without treatment |
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True or false: Endemic typhus is also called Toulon, Mosco, Shop, or Red-Fever Typhus?
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True
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Spotted Fever Groups cause what disease?
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Rocky mountain fever - R. rickettsii
Tick typhus - R conorii Rickettsiapox - R. akari |
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where in the cell are bacteria from the spotted fever group located?
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Nucleus/Cytoplasm
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True or False:
Roxky mountain spotted fever can be transmitted by dermacentor andersoni and dermacentor variabilis? |
True
- Wood Tick - Dog Tick |
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Arthropod Vector Tick, rash appears on ankle/wrist/forhead and spreads to trunk
Guinea Pig - fever, necrosis, skin ulcers, hemorrhagic rash in scrotum Weil-Felix - +4 OX19, + OX2, 0 OXK Disease/Microbe/Treatment |
Rocky Mountain spotted Fever
R. rickettsii Tetracycline/Chloramphenicol |
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Symptoms of RMSF?
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abrupt fever, chills, headache, rash spreads from extremities to trunk, seizures, coma, renal failure, GI problems
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Lesion tache noire, necrotic center, eschar developments, regional lymph nodes enlarged.
Weil-Felix - + OX19, + OX2 Guinea Pig - fever and swelling of the scrotum Bacteria located in nucleus/cytoplasm. Disease/Microbe/Treatment |
Boutonneuse fever/tick Typhus
R. conorii |
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Arthropod vector blood sucking mite
Bacteria loacted in nucleis/cytoplasm No Weil-Felix Rxns Guinea Pig - fever and swelling of scrotum |
Rickettsialpox
R. akari |
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Arthropod vector mite larvae, chiggers
bacteria located in cytoplasm of cells Weil-Felix +4 OXK |
Scrub Typhus
Orientia tsutsugamushi Tetracycline |
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Stable outside of host cells, no arthropod vector, no OX reactions, aerosol transmission.
Obligate Gram - coccobacillus, "spore form" Guinea - fever |
Q Fever
Coxiella burneti Tetracycline |
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Slightly curved gram - rods
Cat - Oxi - Ure - Cultivated on Chocolate Agar with CO2 |
Rochalimaea (Bartonella) quintana
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Arthropod vector is the body louse, no OX reactions, characterized by 3-5 day cycle
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Trench fever
Rochalimaea quintana |
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What causes Cat Scratch Fever?
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Rochalimea (Bartonella) henselae
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Arthropod Vector is Sand Fleas, occurs in Andes Mts., causes Oroya fever/Verruga fever.
What are these? what organism? |
Oroya - hemolytic anemia
Verruga - eruption of skin nodules Two stages of Carrion's disease Rochalimaea (Bartonella) bacilliformis |
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Arthropod vector is dog tick, grows in leukocytes.
Small obligate, intracellular bacteria, cocci-coccobacilli |
Ehrlichiosis
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