Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
35 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
When recovery is used? |
If a bias is found between the test method and reference method, it is used to understand why. |
If a ____ is ____ between the ____ ______ and ____ _____, it is ___ to _____ ____. |
|
|
When recovery is used?
If a bias is ______ between the test _____ and reference ____, it is _____ to understand why? |
_____ bias is found _____ the ______ method and _____ method, it is used to ______ why. |
If a between test reference understand |
|
|
When recovery is used?
If a ____ is found between the ____ method and _____ method, it is used to understand ___. |
If a bias is ____ between the test _____ and reference _______, it is ____ to ______ why? |
found method method used understand |
|
|
When recovery is used?
If a _____ is _____ between the ___________ and _______, it is ___ to ___________. |
_____ bias ___ found _______ test method ____ reference method, _____ used ___ understand why. |
If a is between and it is to |
|
|
When recovery is used? |
If a bias is found between test method and reference method, it is used to understand why.
What is the ideal recovery? |
100% |
|
|
What is the ideal recovery? |
100%
When recovery is used? |
If a bias is found between test method and reference method, it is used to understand why. |
|
|
WHAT IS IDEAL RECOVERY? |
100% What is the usual cause for lower recovery? |
Another substance - reacts with the analyte - competes with reagent |
|
|
What is the usual cause for lower recovery? |
Another substance - reacts with the analyte - competes with reagent |
|
|
|
What is the usual cause for lower recovery? ________ substance - _______ with the analyte - ________ with reagent |
Another ___________ - reacts with _____ - competes with _______ |
substance analyte reagent |
|
|
What is the usual cause for lower recovery? ________________ - _______ with the _______ - _________ with ______ |
Another substance reacts _________ analyte competes ______ reagent |
with the with |
|
|
What is the usual cause for lower recovery? |
Another substance - reacts with the analyte - competes with reagent
Purpose of recovery experiment |
Estimate proportional systematic error |
|
|
Purpose of recovery experiment |
Estimate proportional systematic error |
|
|
|
Purpose of recovery experiment
Estimate ______ systematic _____ |
________ proportional _______ error |
Estimate systematic |
|
|
Purpose of recovery experimetn |
Estimte proportinal systematic error
Notes |
Recall use of slope in MCS Linear regression analysis |
|
|
Notes Factors to consider in recovery exp. |
1. Volume of stock standard added 2. Pipetting accuracy 3. Conc. of analyte added 4. Conc. of standard solution 5. Number of replicates 6. Number of samples to test 7. Comparative method verification |
|
|
|
Factors to consider in recovery exp.
1. __________ (notes) 2. Pipetting accuracy 3. Conc. of analyte added 4. Conc. of standard solution 5. Number of replicates 6. Number of samples to test 7. Comparative method verification |
Volumen of stock standard added
Keep volume of added analyte (water) low to minimize over dilution of oroginal sample Maintain original matrix Recommended: over 10% dilution Ex. 100 ul (0.1 ml) std solution to 900 ul sample |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. ___________ (notes) 3. Conc. of analyte added 4. Conc. of standard solution 5. Number of replicates 6. Number of samples to test 7. Comparative method verification |
Pipetting acccuracy
Since we are calculating conc recovery from a specific starting amount, accuracy is critical Use high quality pipettes |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. Pipetting accuracy 3. ____________ 4. Conc. of standard solution 5. Number of replicates 6. Number of samples to test 7. Comparative method verification |
Conc of analyte added
Good rule of thumb is to add enough analyte to next clinical decision level |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. Pipetting accuracy 3. Conc. of analyte added 4. _____________________ (notes) 5. Number of replicates 6. Number of samples to test 7. Comparative method verification |
Conc of standard solution
Since we want to add at most 10%, make standard 10 times more concentration |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. Pipetting accuracy 3. Conc. of analyte added 4. Conc. of standard solution 5. _____________(notes) 6. Number of samples to test 7. Comparative method verification |
Number of replicates
Duplicate measurements to account for any random error
If low conc. of std added for an analyte, increase to triplicate or quadruplicate measurements |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. Pipetting accuracy 3. Conc. of analyte added 4. Conc. of standard solution 5. Number of replicates 6. __________(notes) 7. Comparative method verification |
Numebr of samples to test
Depends on what interferences might affect full recovery of analyte |
|
|
|
Factors to consider in recovery exp.
1. Volume of stock standard added 2. Pipetting accuracy 3. Conc. of analyte added 4. Conc. of standard solution 5. Number of replicates 6. Number of samples to test 7. ____________ |
comparative method verification
Is the recovery problem in the new test instrumetn or both instruments used in MCS |
|
|
|
Calculation of %recovery |
% recovered= ((measured in spike sample)-(measured in baseline)) / Conc. added
x 100
Notes |
See slide 12 of lecture 7 for ex. |
|
|
Calcultion of % recovery % recovered= ((_______in spike sample)-(______ in baseline)) / Conc. _________
x _____
|
Calcultion of % recovery % _________= ((measured in _______ sample)-(measured in ________)) / ______. added
x 100
|
recovered spike conc 100 |
|
|
Calcultion of % recovery % recovered= ((measured in _________)-(measured in ________)) / ________. added
x _______
|
Calcultion of % recovery % recovered= ((_______ in spike sample)-(_________ in baseline)) / Conc. ________
x 100
|
measured measured added
|
|
|
Calcultion of % recovery % ________= ((________ in ________)-(_______ in ________)) / __________
x _____
|
Calcultion ___ % recovery % recovered= ((measured __ spike sample)__(measured ____ baseline)) __ Conc. added
x 100
|
of in - of / |
|
|
Calculation of % recovery |
Calcultion of % recovery % recovered= ((measured in spike sample)-(measured in baseline)) / Conc. added
x 100 |
|
|
|
How to setup a recovery exp? |
1. Prepare samples 2. Add A (analyte) to one tube, the other is control 3. Measured A in both tubes 4. Calculte % recovery |
|
|
|
How to setup a recovery exp?
1. Prepare _________ 2. Add A (________) to ____ tube, the other is ________ 3. Measured __ in both _____ 4. Calculte %_________ |
How to setup a recovery exp?
1. _________ samples 2. _______(analyte) to one _______, the _________ is control 3. _______ A in _____ tubes 4. __________ recovery |
parepare add a tube other
measured both Calculate % |
|
|
How to setup a recovery exp? |
1. Prepare samples 2. Add A (analyte) to one tube, the other is control 3. Measured A in both tubes 4. Calculte % recovery
Notes |
See slide 17 |
|
|
Review Purpose of recovery experiment |
Estimte proportinal systematic error
How to find this proportional error? |
100%-%recovery |
|
|
How to find proportional error based on recovery? |
100%-recovery |
|
|
|
Consider the CLIA limit for calcium is +/- 1 mg.dl
At the upper level of 10 mg/dl, it has 10% TEa
Assuming no CE and very little RE, what is the performance of this test? |
It just met the CLIA requirement |
|
|
|
Review
Type of error identified by interferecne |
Constant systematic error |
|
|
|
Review
Type of error identified by recovery |
Proportional systematic error |
|
