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14 Cards in this Set
- Front
- Back
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How they isolate Mutants? |
McConkey - P. ??? - sugar eater
Xgal -ve, Bgal (La2) |
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How made 2n ish? Why? |
merodiploid --> cnj F- plasmid --> rembn --> complex array |
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Why compln analysis? (3) |
- # zero prods
- O/R (frn)
- cis / trans |
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What does complementation mean? |
--> (-) --> m1+ m2
on diff genes
--> dominant --> phen = expressed |
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What did they discover about lac z/y rln/hp 2 mlk? |
--> both inducible by mlk |
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Which type of genes did they study to understand regular? |
Milu (inducer) @ sone??? |
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What were the 2 sensitive mutants studied? |
lac1+ Lac0+ |
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What did they detect about LacI? how? i.e. r/d |
LacI = Fec b/c; mtn o?? plmd or dys phenotype was inducible (??) being blocked by WT |
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Is it trans / cis? Why? |
LacI trans - must be a Ptn b/c can act on either O (plmd or chr) in merodipoloid (repressor)
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Gene order? Why? |
IZY mapping
LacO: D: merodiploid phenotypes both constitutive
cis: depends on how other lac genes are: if LacZ mutant, LacOC mtnt also inducible
Why: positional effect reg. site, can't compete I PO Z4 (mapping) |
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What's the operon model then? |
Str1/r1 genes ZY
reg sites --> O
inducer - lactose |
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Mutants predictable? |
Z-Y-, Is, repressor doesnt work but lactose can't bind so NEVER works
P- promoter mtnt |
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Types of activation? |
A pulls RNAP in
alpha bind up elements |
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Crp / cya --> Noninducive? Same merodiploid phenotype type as what? Purpose? |
delta bind sites to match
noninducive, but recessive
gene ??? turned on if ???? is the same as LacI |
