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55 Cards in this Set
- Front
- Back
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Clostridium Difficle |
Anaerobic GI pathogen=?
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Aggulutination test (for toxin A) |
What is the test to ID Clostridium Difficle? |
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Lactose postive |
Pink colonies on Macconkey agar are... ? |
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E. Coli Enterobacter |
List two bacteria that are lactose positive |
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Pseudomonas Proteus Salmonella Shigella |
List four bacteria that are lactose negative |
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Green with black centers |
Color of the Salmonella colonies if on HE agar. |
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Green with black centers |
Color of the Proteus colonies if on HE agar. |
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Green (no black center) |
Color of the colonies if Shigella is present on HE agar. |
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Orange |
Color of the colonies if E. Coli or Enterobacter is present on HE agar |
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Yellow |
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42C 5-6% O2 3-10% CO2 |
Growth of Campylobacter sp. requires what conditions? |
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Red |
Color of XLD agar if Shigella or Pseudomonas is present.
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Yersinia enterocolitica |
YSA agar is used to grow this intestinal pathogen |
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Pink |
Color of Urea agar if Proteus sp is present. |
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Proteus |
bacteria that is associated with "swarming" |
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Salmonella Proteus |
H2S + Fe --> FeS What bacteria would cause this reaction? |
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Hydrolysis of Esculin |
What causes the blacking on Bile Esculin by Enterococcus Faecalis? |
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Enterobacter |
What bacteria produces pink mucoid colonies on Macconkey agar? |
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Yersinia enterocolitica |
Dark pink colonies with a transluscent border on YSA agar at 48 hours of incubation |
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Proteus Salmonella |
Name the two bacteria with red colored colonies with a black center on XLD agar |
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Enterococcus faecalis |
Black colony on Bile Esculin agar |
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E. Coli 0157-H7 |
What is a lactose positive pathogen? |
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Lactose negative |
What type of lactose fermenters are colorless on Macconkey agar? |
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E. Coli |
What bacteria produced flat pink colonies on Macconkey agar? |
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Proteus Psuedomonas |
What two bacteria are lactose negative normal (non-pathogenic) flora? |
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Red with black center |
What color does Salmonella grow on XLD agar? |
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colorless |
What color are Salmonella or Shigella on Macconkey agar? |
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S. enteritidis |
Species of Salmonella that is often associated with eggs? |
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E. coli 0157-H7 |
What is the bacteria that causes HUS? (hemolytic anemia(anemia caused by destruction of red blood cells), acute kidney failure(uremia), and a low platelet count ) |
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selective |
type of media that allows certain types of organisms to grow, and inhibits the growth of other organisms |
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differential |
type of media that contains compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups Ex. BAP -- different hemolysis reactions |
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lactose positive |
type of bacteria that can ferment the carbohydrate (sugar) lactose as a carbon source Ex. E. Coli and Enterobacter |
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lactose negative |
type of bacteria that can ferment the carbohydrate (sugar) lactose as a carbon source Ex. Shigella, Salmonella, Pseudomonas, Proteus |
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Enterobacteriaceae |
large family of Gram-negative rod shaped `bacteria |
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Occult test |
test that is performed to determine if there is blood present in a stool sample |
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Enterococcus faecalis |
gram positive coccus |
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E. Coli |
MAC -- pink flat colonies XLD -- yellow colonies HE -- orange colonies lactose fermenter normal flora of GIT |
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Enterobacter |
MAC -- pink mucoid colonies XLD -- yellow colonies HE -- orange colonies lactose fermenter normal flora of GIT |
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Proteus |
MAC -- colorless XLD -- Red with black center HE -- green with black center possible swarming* H2S producer non-pathogen (normal flora) produce urease -- pink non-lactose fermenter
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Salmonella |
MAC- colorless XLD -- red with black center HE -- green with black center does NOT produce urease (yellow) pathogenic H2S producer non-lactose fermenter |
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Shigella |
MAC -- colorless XLD-- red HE - green does not produce oxidase (not blue) pathogenic non-lactose fermenter |
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Pseudomonas |
MAC -- colorless XLD -- red HE -- green produce oxidase (blue) normal flora non-lactose fermenter |
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Enterococcus faecalis |
gram positive No growth on MAC, XLD, HE (selective gram neg) BAP -- pinpoint white colonies Bile Esculin --- black halos |
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Clostridium difficile |
gram positive rods anaerobic No growth on MAC, XLD, HE (selective gram neg) Takes over GI tract if normal flora is wiped out (antibiotics) |
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GN Broth |
What media promotes growth of Salmonella and Shigella (pathogens) and inhibits growth of normal flora? * selective for gram negative organisms |
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Campylobater jejuni |
Camp agar incubated 24-48 hrs 5-6% O2 (microaerophillic) 3-10% CO2 Gram-negative one of the most common causes of human gastroenteritis |
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cold enrichment |
specimen that thrives in being in colder temps (4C -- refrigeration) ex. Listeria , Yersina |
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Yersinia entercolitica |
Gram-negative bacillus-shaped bacterium cold enriched self-limiting gastrointestinal infections |
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Bile Esculin |
agar that turns black with Enterococcus faecalis selective against gram negative |
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Blood agar |
enriched medium that grows variety of organisms demonstrates hemolytic activity |
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Campylobacter |
selective agar inhibits normal flora selective for Camylobacter jejuni incubated for 24-48 hrs 42C 5-6% O2 3-10% CO2 |
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Hektoen Enteric (HE) |
Green tinted agar Fe present in the agar which reacts with H2S and produces FeS (black) selective for gram negative organisms |
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MacConkey |
selective for gram negative rods differential for lactose fermenting bacteria (pink) non-lactose are colorless
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XLD |
red tinted agar Fe present in the agar which reacts with H2S and produces FeS (black) selective for gram negative organisms |
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Yersinia Selective Agar (YSA) |
inhibits normal flora allows growth of Yersinia enterocolitica (appear dark pink at 48 hours) |
