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27 Cards in this Set
- Front
- Back
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Regulatory mechanism criteria
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nutritional availability trigger
toggle transcription |
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RNA pol
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promoter binding -> transcription start
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operator
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repressor binding site
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genetic switches
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+ activator
- repressor |
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DNA-binding domain
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ptn -- DNA
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allosteric site
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ptn -- allosteric effectors -> binding+
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transcription unit
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coordinately regulated
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lac transcription unit
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Z beta-Gal
Y permease (A xAc) |
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lac operon model
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I --- P - O - [Z - Y - A] ---
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lac repressor
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I -> repressor -| O
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LacI allosteric transition
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chg shape -> decrease repressor-operator affinity
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induction
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inducer -> allosterically inactivate repressor
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partial diploid
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lac F' -> hetero
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constitutive mutation
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not inducer-sensitive
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cis-acting
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regulate same chromosome only
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trans-acting
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regulate on any chromosome
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polar mutation
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affects genes downstream
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Jacob-Monod
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Oc cis, I+ trans => operator-repressor
Is dominant over I+ => allostery Z- => Y- A-, Y- => Z+ A- ==> coordinated regulation |
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catabolite repression
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catabolite prevents metabolic synthesis
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Glu lac control
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Glu catabolite -| ATP -> cAMP
cAMP allosteric effector of CAP cAMP-CAP -| P |
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rotational twofold symmetry
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DNA binding site palindromic
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cAMP-CAP action
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bend O => P-RNA pol
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arabinose control
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allosteric effector arabinose-araC recruits DNA pol to araI
no arabinose => araI-araC-araO loop |
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trp regulation
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O-trp[E-A] in order of pathway catabolism sequence
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trp repression
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trpR -> repressor-trp -| O
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trp leader sequence
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-mRNA O5'-attenuator-trpE
--1--2--3--4-- |
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trp attenuation
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trp => mRNA xlt 1, rib absorb 2 => 3-4 pair => xsc stop => mRNA-
trp- => rib stalled at 1 trp codons => 3,4 open => xsc continues |
