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26 Cards in this Set
- Front
- Back
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Samples were aspirated into tubing to introduce samples into sample holder |
Continuous flow analysis |
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Test reaction can be programmed to occur in a different order than the samples are placed on the instrument |
Continuous flow analyzer |
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Test reaction can be programmed to occur In a different order than the samples are placed on inatrument |
Random access |
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All samples are processed in the order they are received and multiple test are receive |
Sequential analysis |
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These analyzer capable of performing a batch of one type of test to completion |
Centrifugal analysis |
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Agglutinin (soluble antibody ) forms lattice with particulate(insoluble) antigen |
Agglutinin |
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Methods of enhancing agglutination |
Centrifugation Treatment with proteolytic enzymes Use of colloids AHG |
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First phase ,physical attachment of antibody molecules to antigen |
Sensitization |
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Establishment of cross-linking between sensitized particles and antibodies resulting to clumping |
Lattice formation |
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Antigen is naturally bound on surface of cells |
Direct agglutination |
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How many fold of dilution tested for direct agglutination |
Two fold dilution of serum tested |
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Examples of test use direct agglutination |
Febrile agglutination Hemagglutination |
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Detect antibodies to erythrocyte antigens |
Hemagglutination |
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Antibodies do not directly agglutinate rbc |
Hemagglutination |
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Hemagglutination may be detected using ? |
Enhancement medium(AHG) |
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Erythrocyte are coated with substances such as extract of bacterial cells |
Indirect /passive hemagglutination |
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What are the carriers for detecting and titrating antibodies |
Erythrocyte of animals-sheep or rabbit Group o humans |
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Examples of assay performed by indirect hemagglutination |
Antinuclear ribonucleoprotein Anti SM Antithyroglobulin and antithyroid globulin Rubella ab Sheep cell agg titer |
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Antigen are bound to carrier particles |
Passive agglutination |
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Example of passive agg |
Latex ,charcoal,bentonite ,rbc |
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Carrier particle is coated with ag |
Passive agglutination |
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Detect for the presence of abs |
Passive |
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Antibody molecule bound to the surface of latex beads to which particular antigen binds |
Latex agglutination test |
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Examples for latex agglutination test |
ASO latex agglutination test Rubella antibody |
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Based on interaction of soluble antigen with ab that result to formation of ppt particle |
Flocculation test |
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Example of flocculation test |
Venereal disease research laboratory Rapid plasma reagin |
