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10 Cards in this Set
- Front
- Back
- 3rd side (hint)
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What is pre-transfusion testing? |
Pre-transfusion tests include ABO and Rh typing of the recipient's red blood cells (RBC) and an antibody screen. |
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Purpose of pre-transfusion testing? |
– Avoid risks to donor and recipient – Meet product specifications |
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What is the first step in pre-transfusion testing? |
ABO&Rh Typing -testing the recipient RBC for the presence of A and B antigens using anti-A and anti-Bantisera (forward grouping). -Testing of the recipient plasma for the presence of anti-A and anti-B using known group A and group B cells (reverse grouping). |
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Next Step? |
Antibody Screening -sample of the patient'splasma or serum is tested against selected group O RBC that bear most or all clinically significant antigens |
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After screening, antibody identiification is carried out. What is the differences between Ab screening and identification? |
Screening cells -Antibody detection -Sets of 2 or 3 vials Panel cells -Antibody identification -At least 10 vials per set |
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What is the last step in pre-trasfusion testing? |
Cross-matching -a method of confirming compatibility between the patient'sblood (plasma) and the donor RBCs -direct mixing of donor RBCs with recipientplasma (serological crossmatch) -electronic or computer-assisted crossmatch |
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What type of cells are tested in a panel? |
O negative cells |
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Name 3 ways to resolve incompatibilities in the crossmatch? |
1) resolve pos result by ruling out ABO incompatibility 2) perform RT panel to ID presence of cold reactive antibody 3) Resolve rouleaux by repeating IS crossmatch with saline replacement technique |
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What is the Rule of Three? |
If plasma reacts with 3 antigen positive cells and does not react with 3 antigen negative cells, there is a 95% probability the identification did not happen by chance. |
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What is the autocontrol in Ab identification indicating? |
Negative- alloantibody Positive– autoantibody or DTR (i.e.,alloantibodies) |
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